Regulatory mechanism of replication complex formation and relationship between the complex, checkpoints and cell division

染色体DNA复制受到细胞分裂周期的严格调控，因此染色体DNA在每个细胞周期复制一次。这主要是因为细胞周期蛋白依赖的蛋白激酶(CDK)是一种蛋白质磷酸化酶，调节着染色体DNA复制的起始步骤。利用发芽酵母，我们发现CDK磷酸化SLD2和SLD3蛋白，这两种磷酸化蛋白都与Dpb11蛋白结合，这些结合促进DNA复制。我们还发现了这些结合如何作用于DNA复制的线索。

Chromosome DNA replication is strictly regulated by the cell division cycle, so that chromosome DNA replicates once per cell cycle. This is mainly because the cyclin-dependent protein kinase (CDK), a protein phosphorylating enzyme, regulates the initiation step of chromosome DNA replication. Using budding yeast, we revealed that CDK phosphorylates Sld2 and Sld3 proteins, both phosphorylated proteins bind to the Dpb11 protein and these bindings promote DNA replication. We also found a clue how these bindings function for the initiation of DNA replication.