Development of a light detection system for enhanced scanning electron microscopy imaging of fully hydrated biological specimens at room temperature.

开发光检测系统，用于在室温下对完全水合的生物样本进行增强扫描电子显微镜成像。

• 批准号: LP0562159
• 负责人: Prof Matthew Phillips
• 金额: $ 16.04万
• 依托单位: University of Technology Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-08-14 至 2009-08-14
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0562159
• 关键词: Development; light; detection; system; enhanced

This project aims to develop instrumentation and techniques to image fully hydrated cellS for prolonged periods at room temperature in an environmental scanning electron microscope. Multiple fluorescent nano-particle labels will be used to image complex cellular structure and dynamics at ultra high spatial resolution. This innovative bio-imaging technique will be facilitated by the construction of a high speed light collection system that can efficiently operate at the pressures required to stablise water within the cell. Applications for this new bio-imaging technique will also be explored.

该项目旨在开发仪器和技术，在室温下在环境扫描电子显微镜下长时间成像完全水合的细胞。多个荧光纳米粒子标签将用于成像复杂的细胞结构和动态在超高空间分辨率。这种创新的生物成像技术将通过高速光收集系统的建设而得到促进，该系统可以在稳定细胞内水分所需的压力下有效地运行。此外，还将探讨这种新型生物成像技术的应用。