Development of cell adhesive factor coated-implant having high bone inductive ability.

开发具有高骨诱导能力的细胞粘附因子涂层植入物。

• 批准号: 09557157
• 负责人: AKAGAWA Yasumasa
• 金额: $ 7.3万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557157/
• 关键词: Implant; Hydroxyapatite; Titanium; Osteoblast; Cell adhesion; RGD peptide; Specific adhesion

Hydroxyapatite (HA) is a bloactive dental implant material which accelerates bone formation on its surface. The mechanism of this acceleration is not clear. The elucidation of the cell adhesion might be the key to understanding the bioactive mechanism of HA.In this study, we analyzed the adhesion of HOS human osteoblasts onto HA and titanium to find out the particular adhesion to HA.In short-term cultures in fetal bovine serum-precoated materials, a significantly higher number of cells adhered to HA than to titanium. In addition, serum-free conditions with phosphate buffered-saline precoating or bovine serum albumin precoating materials were tested. The results were nearly the same among all precoating conditions, suggesting that the quantity of cell adhesion was not affected by serum components. However, in the morphological observation by SEM, the form of adhesion was found to differ among precoating conditions. The osteoblasts tightly adhered and spread onto both HA and titanium with serum precoating, whereas the cells loosely adhered and did not spread without serum. To evaluate the Arg-Gly-Asp (RGD) sequence-specific adhesion, synthetic RGD peptides were used for a competitive inhibition test. The result showed that RGD peptides remarkably inhibited the tight adhesion and spreading of osteoblasts onto HA, whereas it did not strongly inhibit these onto titanium. These results demonstrate that the regulation of cell adhesion to HA is different from that to titanium. Our study suggests that the RGD-containing serum proteins might have a major role in regulating the specific adhesion of osteoblasts to [IA, and in inducing enhanced cell growth and differentiation.

羟基磷灰石 (HA) 是一种膨胀性牙科植入材料，可加速其表面的骨形成。这种加速的机制尚不清楚。细胞粘附的阐明可能是理解HA生物活性机制的关键。在本研究中，我们分析了HOS人成骨细胞对HA和钛的粘附，以找出对HA的特殊粘附。在胎牛血清预涂材料的短期培养中，粘附到HA的细胞数量明显高于粘附到钛的细胞数量。此外，还测试了磷酸盐缓冲盐水预涂或牛血清白蛋白预涂材料的无血清条件。所有预涂条件下的结果几乎相同，表明细胞粘附量不受血清成分的影响。然而，在通过SEM进行的形态观察中发现，根据预涂条件的不同，粘附的形式也不同。成骨细胞紧密粘附并扩散到具有血清预涂的HA和钛上，而细胞则松散粘附并且在没有血清的情况下不扩散。为了评估 Arg-Gly-Asp (RGD) 序列特异性粘附，使用合成的 RGD 肽进行竞争性抑制测试。结果表明，RGD肽显着抑制成骨细胞在HA上的紧密粘附和铺展，而在钛上则没有强烈抑制。这些结果表明，HA 的细胞粘附调节与钛的细胞粘附调节不同。我们的研究表明，含有 RGD 的血清蛋白可能在调节成骨细胞与 [IA 的特异性粘附，以及诱导增强的细胞生长和分化方面发挥重要作用。

项目成果

K.Okamoto et al.: "RGD peptides regulate the specific adhesion scheme of osteoblasts to hydroxyapatite but not to titanium" Journal of dental research. 77・3. 481-487 (1998)

K. Okamoto 等人：“RGD 肽调节成骨细胞对羟基磷灰石的特异性粘附方案，但不调节对钛的特异性粘附方案”牙科研究杂志 77・3 481-487 (1998)。

K.Okamoto, T.Matsuura, R.Hosokawa and Y.Akagawa: "RGD peptides regulate the specific adhesion scheme of osteoblasts to hydroxyapatite but not to titanium" Journal of dental reserch. 77(3). 481-487 (1998)

K.Okamoto、T.Matsuura、R.Hosokawa 和 Y.Akakawa：“RGD 肽调节成骨细胞对羟基磷灰石的特异性粘附方案，但不调节对钛的特异性粘附方案”牙科研究杂志。