In vivo physical and biological action of new antimicrobial tissue conditioner

新型抗菌组织调理剂的体内物理和生物作用

• 批准号: 12470420
• 负责人: AKAGAWA Yasumasa
• 金额: $ 6.91万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470420/
• 关键词: tissue conditioner; antimicrobial; respiratory infections; denture stomatitis; silver-zeolite

Tissue conditioner (TC) is readily degradable and susceptible to colonization by microorganisms. Unless a tissue conditioner is replaced regularly, it acts as a reservoir for microorganisms and can cause complications. In elderly patients who have decreased immunological activity, such complications, including pharyngeal and respiratory tract problems, are inevitable when microorganisms have accumulated on the tissue conditioners. Silver-zeolite has been shown to possess long-term antimicrobial effects against almost all microbes, and has given the continuous release of small amount (ca. 10 ppb) of silver ions into water. We tried to develop a new antimicrobial tissue conditioner containing silver-zeolite.Two TCs containing 0 and 2 % -SZ were prepared and a plastic dish was used as a control. Candida albicance and nosocomial respiratory infection-causing bacteria, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Streptococcus constellat … More us and Streptococcus intermedius were selected as test microorganisms. Microbial suspension was incubated at 37 ℃ for 24 hrs on each TC disk after water or saliva immersion for 28 days. Antimicrobial effects of TCs were evaluated by counting the number of viable cells (CFU) in the suspension. In addition, after 7-days-saliva-immersed samples with or without SZ were chemically cleansed with each solution of three denture cleansers, the antimicrobial effects of those against P. aeruginosa were evaluated. The antimicrobial effects of TCs with SZ immersed in saliva for 28 days against C. albicance, S. aureus and MRSA were observed, while CFU of P. aeruginosa indicated no significant change and CFU of the S. milleri group were significantly reduced in comparison with that of the control. As for P. aeruginosa, after TC with SZ immersed in saliva for 7 days was treated with the denture cleansers, its antimicrobial effect was detected again.These results suggest that TC containing is a possible candidate for a novel antimicrobial tissue conditioner. Less

组织调理剂（TC）易于降解，易受微生物定植。除非定期更换组织护发素，否则它会成为微生物的储存库，并可能导致并发症。在免疫活性下降的老年患者中，当微生物在组织调理剂上积累时，诸如咽部和呼吸道问题等并发症是不可避免的。银沸石已被证明对几乎所有微生物都具有长期的抗菌作用，并能向水中持续释放少量（约10 ppb）银离子。我们试图开发一种含有银沸石的新型抗菌组织护发素。制备了两种含0和2% -SZ的TCs，并用塑料培养皿作为对照。选取白色念珠菌和院内呼吸道致病菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌、星形链球菌等作为试验微生物。水或唾液浸泡28 d后，在每个TC盘上37℃孵育24 h。通过计算悬液中活菌数（CFU）来评估TCs的抗菌作用。此外，用三种义齿清洁剂溶液分别清洗含SZ或不含SZ的唾液浸泡样品7天后，评估其对铜绿假单胞菌的抗菌效果。唾液中浸没SZ 28 d的TCs对白色假单胞菌、金黄色葡萄球菌和MRSA的抑菌效果观察，P. aeruginosa的CFU无明显变化，而S. milleri组的CFU较对照组明显降低。对于铜绿假单胞菌（P. aeruginosa），将SZ浸入唾液7天后，用义齿清洁剂处理TC，再次检测其抗菌效果。这些结果表明，含TC可能是一种新型抗菌组织调理剂的候选物。少