In vivo physical and biological action of new antimicrobial tissue conditioner

组织调节剂(TC)很容易被微生物降解和定植。除非定期更换组织调节剂，否则它会成为微生物的储存库，并可能导致并发症。在免疫活性降低的老年患者中，当组织调节剂上积累了微生物时，这种并发症是不可避免的，包括咽部和呼吸道问题。银沸石已被证明对几乎所有的微生物具有长期的抗菌作用，并使少量(约10 ppb)的银离子持续释放到水中。我们试图开发一种新型的含银沸石的抗菌组织调理剂，制备了两种含0%和2%-SZ的TCS，并以塑料培养皿为对照。白色念珠菌和医院内呼吸道感染致病菌金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌、星状链球菌…选择了较多的US和中间链球菌作为试验微生物。在水或唾液浸泡28天后，将微生物悬液在每个TC盘上37℃孵育24小时。通过计数悬液中活细胞数(CFU)评价TCS的抗菌效果。此外，将含或不含SZ的唾液浸泡7天后，分别用三种义齿清洁剂的溶液进行化学清洗，评价其对铜绿假单胞菌的抑菌效果。唾液浸泡SZ 28天的TCS对白色念珠菌、金黄色葡萄球菌和金黄色葡萄球菌的抑菌效果与对照组相比差异无统计学意义，而对铜绿假单胞菌的菌落数无明显影响，米勒葡萄球菌组的菌落数明显低于对照组。对于铜绿假单胞菌，用义齿清洁剂处理浸泡在唾液中7天的TC后，再次检测到其抗菌效果，这些结果表明，含TC的TC可能是一种新型抗菌组织调节剂。较少

Tissue conditioner (TC) is readily degradable and susceptible to colonization by microorganisms. Unless a tissue conditioner is replaced regularly, it acts as a reservoir for microorganisms and can cause complications. In elderly patients who have decreased immunological activity, such complications, including pharyngeal and respiratory tract problems, are inevitable when microorganisms have accumulated on the tissue conditioners. Silver-zeolite has been shown to possess long-term antimicrobial effects against almost all microbes, and has given the continuous release of small amount (ca. 10 ppb) of silver ions into water. We tried to develop a new antimicrobial tissue conditioner containing silver-zeolite.Two TCs containing 0 and 2 % -SZ were prepared and a plastic dish was used as a control. Candida albicance and nosocomial respiratory infection-causing bacteria, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Streptococcus constellat … More us and Streptococcus intermedius were selected as test microorganisms. Microbial suspension was incubated at 37 ℃ for 24 hrs on each TC disk after water or saliva immersion for 28 days. Antimicrobial effects of TCs were evaluated by counting the number of viable cells (CFU) in the suspension. In addition, after 7-days-saliva-immersed samples with or without SZ were chemically cleansed with each solution of three denture cleansers, the antimicrobial effects of those against P. aeruginosa were evaluated. The antimicrobial effects of TCs with SZ immersed in saliva for 28 days against C. albicance, S. aureus and MRSA were observed, while CFU of P. aeruginosa indicated no significant change and CFU of the S. milleri group were significantly reduced in comparison with that of the control. As for P. aeruginosa, after TC with SZ immersed in saliva for 7 days was treated with the denture cleansers, its antimicrobial effect was detected again.These results suggest that TC containing is a possible candidate for a novel antimicrobial tissue conditioner. Less