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Adherence of periodontal ligament cells to dental implant materials

牙周膜细胞与牙种植材料的粘附

基本信息

批准号：
07457471
负责人：
AKAGAWA Yasumasa
金额：
$ 4.74万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Modern dental implants obtain a direct bonding to bone, osseointegration and are accepted to have long-term clinical success. However, these osseointegrated implants have no senser and no stress absorber like periodontal ligament, still having some problems about the control of mandibular movement and occlusal stress. With regard to the occlusal function, the ideal dental implants may be ones with periodontal ligaments. So far no in vitro and in vivo studies have been performed to pioneer periodontal ligament implant. In addition, periodontal ligament cells have not been characterized yet.We first developed a serum-free medium for the growth and differentiation of periodontal ligament cells and study the effect of fibroblast growth factors (FGFs) on the growth and differentiation. Secondly, the adherence of osteoblasts, that well relate to periodontal ligament cells, to hydroxyapatite and titanium was investigated.Periodontal ligament cells express FGF-1, FGF-2 and FGF receptor-1 during growth and differention, suggesting that FGF-1 and FGF receptor-1 have a main role of regulating the growth and differentiation of the cells. Further the adherence of osteoblasts to hydroxyapatite and titanium is mediated by adhesive protein RGD domains.This study shows that specific interaction by FGFs and adhesive proteins may be useful to achieve periodontal ligament implant.

现代牙科种植体获得与骨的直接结合，骨整合，并被接受具有长期的临床成功。然而，这些骨结合种植体没有牙周膜那样的传感器和应力吸收器，在下颌运动和咬合应力的控制方面仍然存在一些问题。就咬合功能而言，理想的种植体可能是带有牙周韧带的种植体。迄今为止，还没有体外和体内研究已经进行了先驱牙周韧带植入物。另外，牙周膜细胞的特性尚不清楚，本研究首先建立了牙周膜细胞生长分化的无血清培养基，并研究了成纤维细胞生长因子（fibroblast growth factors，FGFs）对牙周膜细胞生长分化的影响。其次，研究了与牙周膜细胞密切相关的成骨细胞与羟基磷灰石和钛的粘附情况，牙周膜细胞在生长分化过程中表达FGF-1、FGF-2和FGF受体1，提示FGF-1和FGF受体1在调节细胞生长分化中起主要作用。此外，成骨细胞与羟基磷灰石和钛的粘附是由粘附蛋白RGD结构域介导的，本研究表明，FGF与粘附蛋白的特异性相互作用可能有助于牙周膜植入的实现。