Adherence of periodontal ligament cells to dental implant materials

现代牙种植体直接与骨结合，与骨结合，在临床上取得长期成功。然而，这些骨整合种植体没有像牙周韧带那样的感应器和应力吸收装置，在控制下颌运动和咬合应力方面仍存在一些问题。就咬合功能而言，理想的种植体可能是有牙周韧带的种植体。到目前为止，还没有体外和体内的研究来开创牙周膜种植的先河。此外，牙周膜细胞的特性尚未得到证实。我们首次建立了牙周膜细胞生长分化的无血清培养体系，并研究了成纤维细胞生长因子(FGFs)对牙周膜细胞生长分化的影响。其次，对与牙周韧带细胞密切相关的成骨细胞在羟基磷灰石和钛上的黏附进行了研究，发现牙周韧带细胞在生长分化过程中表达成纤维细胞生长因子-1、成纤维细胞生长因子-2和成纤维细胞生长因子受体-1，提示成纤维细胞生长因子-1和成纤维细胞生长因子受体-1在调控细胞生长和分化中起主要作用。此外，成骨细胞对羟基磷灰石和钛的黏附是由黏附蛋白RGD结构域介导的。这项研究表明，FGFs和黏附蛋白的特异性相互作用可能有助于实现牙周韧带植入。

Modern dental implants obtain a direct bonding to bone, osseointegration and are accepted to have long-term clinical success. However, these osseointegrated implants have no senser and no stress absorber like periodontal ligament, still having some problems about the control of mandibular movement and occlusal stress. With regard to the occlusal function, the ideal dental implants may be ones with periodontal ligaments. So far no in vitro and in vivo studies have been performed to pioneer periodontal ligament implant. In addition, periodontal ligament cells have not been characterized yet.We first developed a serum-free medium for the growth and differentiation of periodontal ligament cells and study the effect of fibroblast growth factors (FGFs) on the growth and differentiation. Secondly, the adherence of osteoblasts, that well relate to periodontal ligament cells, to hydroxyapatite and titanium was investigated.Periodontal ligament cells express FGF-1, FGF-2 and FGF receptor-1 during growth and differention, suggesting that FGF-1 and FGF receptor-1 have a main role of regulating the growth and differentiation of the cells. Further the adherence of osteoblasts to hydroxyapatite and titanium is mediated by adhesive protein RGD domains.This study shows that specific interaction by FGFs and adhesive proteins may be useful to achieve periodontal ligament implant.