NADH FLUORESCENCE IMAGING IN HEART

这项研究的长期目标是开发量化的 组织生物能量学研究的光学成像方法。同相 一、研制了一种高空间分辨率的视频荧光仪 它产生的NADH荧光图像补偿了内部 由于血液容量的变化而被吸收。这个装置被用来 在缓冲液和血液中评估局部和全脑缺血 灌流的老鼠心。在第一阶段期间进行的初步研究 证明增强的仪器可以产生免费的 使用独特的钯卟啉的组织氧分压图像。 第二阶段将包括：(1)将视频荧光计升级为 获得顺序的NADH和钯的卟啉发射图像， (2)合适的钯-卟啉化合物的合成及评价 它们在体内使用的效用，(3)开发软件来生产 组织氧浓度图像，以及(4)校准和评估 这种增强型视频荧光仪在模型研究中的应用 缺氧和缺血。 这种处于第二阶段的双功能视频荧光仪将允许快速、 组织代谢和氧浓度的序贯成像 因此在对组织进行空间量化方面具有更大的实用性 新陈代谢和研究用氧供应/需求 潜在的临床用途比现有的设备。

The long term goal of this research is to develop quantitative optical imaging methods for tissue bioenergetic studies. In Phase I, a videofluorometer with high spatial resolution was developed that produces NADH fluorescence images compensated for internal absorption due to blood volume changes. This device was used to evaluate both local and global ischemia in buffer and blood perfused rat hearts. Initial studies performed during Phase I demonstrate that an enhanced instrument can produce complimentary images of tissue oxygen tension using unique palladium porphyrins. Phase II will consist of: (1) upgrading the videofluorometer to obtain sequential NADH and palladium porphyrin emission images, (2) synthesizing appropriate palladium porphyrins and evaluating their utility for in vivo use, (3) developing software to produce tissue oxygen concentration images, and (4) calibrate and evaluate the use of this enhanced videofluorometer in studies of models of hypoxia and ischemia. This dual function videofluorometer in Phase II will allow rapid, sequential imaging of tissue metabolism and oxygen concentration and thus have much greater utility in spatially quantifying tissue metabolism and oxygen supply/demand for research purposes and potential clinical uses than current devices.