Preparation of tailor-made artificial receptors to drugs and their analytical applications

药物定制人工受体的制备及其分析应用

• 批准号: 09672196
• 负责人: TAKEUCHI Toshifumi
• 金额: $ 2.37万
• 依托单位: Hiroshima City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672196/
• 关键词: artificial receptors; molecular imprinting; drugs; 多点水素結合

In this project, molecularly imprinted polymers were prepared for drugs using novel functional monomers in order. to develop new molecular recognition based analytical systems. The summary of the project is as follows.1) Imprinted polymers were prepared for a anti cancer agent, 5-fluorouracil, using a newly developed functional monomer, 2,6-bis(acrylamido)pyridine. The selectivity for 5-fluorouracil was insufficient, however, the usefulness of the functional monomer, 2,6-bis(acrylamido) pyridine for nucleic acid bases was demonstrated.2) Cinchona alkaloids were imprinted using methacrylic acid and/or 2-(trifluoromethyl)acrylic acid and it appeared that for the diastereoselective binding methacrylic acid was suitable for the imprinting of cinchonidine and 2-(trifluoromethyl)acrylic acid was better for the imprinting of the antipod, cinchonine.3) By using a Zn(II)porphyrin monomer as a new functional monomer, a nucleic acid base derivative, 9-ethyl adenine was imprinted and the resultant … More polymer exhibited the selective binding behavior for the adenine structure. The binding is due to the metal complexation, therefore this system may be work both in organic solvents and aqueous solution. The metalloporphyrin-based polymer gave a lambdamax shift (red shift) and fluorescence of the polymer was quenched, according to the complexation with 9-ethyl adenine. This suggests that the molecular recognition based specific binding could be read-out by measuring the polymer's spectroscopic behaviors, and this may open a new strategy of specific detection systems for nucleic acid bases.4) Castasterone, a plant hormone was imprinted using p-vinylbenzene boronic acid as a functional monomer. The resulting polymer showed a high selectivity for castasterone.5) Indol acetic acid-imprinted polymers were prepared using N,N-dimethylaminoethyl methacrylate as a functional monomer and the polymer was applied to solid-phase extraction of indol acetic acid. With the use of chloroform as a extraction solvent, more than 90 % of indol acetic acid was recovered. Less

本课题以新型功能单体为原料，依次制备药物分子印迹聚合物。开发新的基于分子识别的分析系统。本课题的主要内容如下：1）以新型功能单体2，6-双丙烯酰胺基吡啶为模板分子，制备了抗癌药物5-氟尿嘧啶的分子印迹聚合物。对5-氟尿嘧啶的选择性不足，然而，官能单体2，6-双（丙烯酰胺基）吡啶对核酸碱基的印迹作用。2）以甲基丙烯酸和/或2-甲基丙烯酰胺为模板分子，对金鸡纳生物碱进行分子印迹（三氟甲基）丙烯酸，并且似乎对于非对映选择性结合，甲基丙烯酸适合于辛可尼定和2-（三氟甲基）丙烯酸的印迹。3）以锌卟啉为功能单体，对核酸碱基衍生物9-乙基腺嘌呤进行了分子印迹，并对印迹产物进行了表征 ...更多信息 聚合物表现出对腺嘌呤结构的选择性结合行为。结合是由于金属络合，因此该系统可以在有机溶剂和水溶液中工作。根据与9-乙基腺嘌呤的络合作用，基于金属卟啉的聚合物产生了λ max位移（红移），并且聚合物的荧光被猝灭。这表明，基于分子识别的特异性结合可以通过测量聚合物的光谱行为来读出，这可能为核酸碱基的特异性检测系统开辟一条新的策略。4）以对乙烯基苯硼酸为功能单体，对植物激素Castasterone进行分子印迹。5）以甲基丙烯酸二甲氨基乙酯为功能单体，制备了吲哚乙酸分子印迹聚合物，并将其应用于吲哚乙酸的固相萃取。用氯仿作萃取剂，吲哚乙酸的回收率可达90%以上。少

项目成果

竹内 俊文: "レセプターを用いる薬物の定量-天然及び人工レセプターを利用して-" 臨床化学. 26. 1-6 (1997)

Toshifumi Takeuchi：“使用受体进行药物定量 - 利用天然和人工受体 -”临床化学 26. 1-6 (1997)。

Takeuchi, T.: "Molecular imprinting. Tailor-made synthetic receptors capable of molecular recognition." Tanpakushitsu Kakusan koso. 42. 1320-1326 (1997)

Takeuchi, T.：“分子印记。能够进行分子识别的定制合成受体。”

Matsui, J., Tachibana, Y., Takeuchi, T.: "Molecularly Imprinted Receptor having Metalloporphyrin-based Signaling Binding Site." Anal.Commun.35. 225-227 (1998)

Matsui, J.、Tachibana, Y.、Takeuchi, T.：“具有基于金属卟啉的信号传导结合位点的分子印迹受体。”

Takeuchi, T.: "Determination of drugs using natural/synthetic receptors." Rinsho Kagaku. 26. 1-6 (1997)

Takeuchi, T.：“使用天然/合成受体测定药物。”

Kugimiya, A., Matsui, J., Abe, H., Aburatani, M., Takeuchi, T.: "Synthesis of Castasterone Selective Polymers Prepaired by Molecular Imprinting" Anal.Chim.Acta. 365. 75-79 (1998)

Kugimiya, A.、Matsui, J.、Abe, H.、Aburatani, M.、Takeuchi, T.：“通过分子印迹制备的卡斯特酮选择性聚合物的合成”Anal.Chim.Acta。