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Imprinted Potymer based Sensor Array Plate

基于压印聚合物的传感器阵列板

基本信息

批准号：
13672260
负责人：
TAKEUCHI Toshifumi
金额：
$ 2.62万
依托单位：
Kobe University (2002)Hiroshima City University (2001)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672260/
关键词：
molecular imprinting molecular recognition metallo-porphyrins sensing 鉄ポルフィリン

项目摘要

1. A diastereoselective molecubrly imprinted polymer (MIP) for (-) cinchonidine, PPM (CD), was prepared by the combined use of methacrylic acid and vinyl-substituted metallo-porphyrin as functional monomers. Compared to MIPs using only methaciylic acid or metallo-porphyrin as a functional monomer, PM (CD) and PP (CD), respectively, PPM (CD) showed higher binding ability for (-) -cinchonidine in chromatographic tests using the MIPs-packed columns. The MIPs containing a metallo-porphyrin in the binding sites, PPM (CD) and PP (CD), showed spectroscopic change according to the binding of (-) -cinchonidine and it was significant in the low concentration range, suggesting that the high affinity binding sites contain the porphyrin residue. The correlation of the spectroscopic change against log of (-) -cinchonidine concentrations showed a linear relationship. These results revealed that the MIP having highly specific binding sites was assembled by the functional monomers, vinyl-substituted metallo-porphyrin and methacrylic acid, and they cooperatively worked to yield the specific binding. In addition, the metallo-porphyrin-based MIPs appeared to act as a sensor selectively responded by binding events of the template molecule.2. A molecularly imprinted polymer library was prepared on a 96-well microtiter plate using a liquid-handing equipment that was programmed to prepare various breeds of cinchonidine-imprinted polymers. The resultant polymers immobilized on the bottom surface of the wells were screened for the binding capacity by fluorescence measurement using a microplate reader. Comparison with a conventional assessment by FIA demonstrated the reliability and the high-throughput performance of the microtiter plate-based combinatorial molecular imprinting.

1. 以甲基丙烯酸和乙烯基取代金属卟啉为功能单体，制备了（-）辛可尼定非对映选择性分子印迹聚合物（PPM (CD)）。与仅使用甲基乙酸或金属卟啉作为功能单体的MIPs相比，PM （CD）和PP （CD）在使用MIPs填充柱的色谱测试中显示出更高的(-)-辛可尼定结合能力。结合位点PPM （CD）和PP （CD）中含有金属卟啉的MIPs，根据(-)-cinchonidine的结合表现出光谱变化，且在低浓度范围内变化显著，表明高亲和力结合位点含有卟啉残基。光谱变化与(-)-辛可尼定浓度对数呈线性关系。这些结果表明，具有高度特异性结合位点的MIP是由功能单体、乙烯基取代金属卟啉和甲基丙烯酸组装而成的，它们协同作用产生了特异性结合。此外，基于金属卟啉的mip似乎作为传感器选择性地响应模板分子的结合事件。在96孔微滴板上，利用液体处理设备制备了分子印迹聚合物文库，该设备可制备不同品种的辛可尼定印迹聚合物。所得到的聚合物固定在井的底表面上，通过荧光测量使用微孔板阅读器筛选结合能力。通过与传统分子印迹法的比较，验证了该方法的可靠性和高通量性能。