Combinatorial Molecular Imprinting for the Preparation of Synthetic Polymers Capable of Molecular Recogniti

组合分子印迹制备具有分子识别能力的合成聚合物

• 批准号: 11672151
• 负责人: TAKEUCHI Toshifumi
• 金额: $ 1.98万
• 依托单位: Hiroshima City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672151/
• 关键词: Molecular Imprinting; Molecular recognition; Combinatorial Chemistry; 蛍光検出; マイクロプレート; コンビナトリアルMI法

In this study, an automated system for molecularly imprinted polymer synthesis and evaluation was examined. In 1999, molecular imprinting for atrazine and ametryn was performed as an example with the use of various concentration of methacrylic acid and 2-(trifluoromethyl) acrylic acid as functional monomers. It appeared that methacrylic acid was suitable for atrazine imprinting and for ametryn imprinting 2-(trifluoromethyl) acrylic acid gave a better selectivity. These results were consistent with the previously reported results of conventional preparation methods, suggesting that the proposed combinatorial molecular imprinting would be feasible for the development of molecularly imprinted polymers. In 2000, a micro titer plate- based method was developed in combinatorial molecular imprinting. By using this method, the evaluation time was extremely shortened and the combinatorial approach became to be more feasible.

在这项研究中，研究了一种用于分子印迹聚合物合成和评价的自动化系统。1999年，以不同浓度的甲基丙烯酸和2-三氟甲基丙烯酸为功能单体，对阿特拉津和阿霉净进行了分子印迹实验。结果表明，甲基丙烯酸适用于阿特拉津印迹，2-三氟甲基丙烯酸对阿特拉津印迹有较好的选择性。这些结果与以前报道的传统制备方法的结果一致，表明组合分子印迹技术在分子印迹聚合物的开发中是可行的。2000年，一种基于微滴定板的组合分子印迹方法被开发出来。该方法极大地缩短了评价时间，使组合方法变得更加可行。

项目成果

Takeuchi,T.,Matsui,J.: "Miniaturized Molecularly Imprinted Continuous Polymer Rods"Journal of High Resolution Chromatography. 23. 44-46. (2000)

Takeuchi,T.,Matsui,J.：“小型化分子印迹连续聚合物棒”高分辨率色谱杂志。

Matsui, J., Higashi, M., Takeuchi, T.: "Molecularly Imprinted Polymer as 9-Ethyladenine Receptor Having a Porphyrin-based Recognition Center"J.Am.Chem.Soc.. 122. 5218-5219 (2000)

Matsui, J.、Higashi, M.、Takeuchi, T.：“具有基于卟啉的识别中心的作为 9-乙腺嘌呤受体的分子印迹聚合物”J.Am.Chem.Soc.. 122. 5218-5219 (2000)

Takeuchi, T., Matsui, J.: "Miniaturized Molecularly Imprinted Continuous Polymer Rods"J.High Resolution Chromatogr.. 23. 44-46 (2000)

Takeuchi, T., Matsui, J.：“小型化分子印迹连续聚合物棒”J.高分辨率色谱. 23. 44-46 (2000)

Takeuchi,T.,Fukuma,D.,Matsui,J.: "Combinatorial Molecular Imprinting : An Approach to Synthetic Polymer Receptors"Analytical Chemistry. 71. 285-290. (1999)

Takeuchi,T.、Fukuma,D.、Matsui,J.：“组合分子印迹：合成聚合物受体的方法”分析化学。

Matsui, J., Kubo, H., Takeuchi, T.: "Molecularly Imprinted Fluorescent-Shift Receptors Prepared Using 2-(Trifluoromethyl) acrylic Acid"Anal.Chem.. 72. 3286-3290 (2000)

Matsui, J.、Kubo, H.、Takeuchi, T.：“使用 2-(三氟甲基)丙烯酸制备的分子印迹荧光位移受体”Anal.Chem.. 72. 3286-3290 (2000)