STUDIES ON PROTECTION AND CONTROL OF EPIDEMIC HEMORRHAGIC FEVER (EHF) IN LABORATORY ANIAML EXPERIMENTATION

实验室动物实验中流行性出血热（EHF）的防护与控制研究

• 批准号: 59380005
• 负责人: YAMANOUCHI TAKAHISA
• 金额: $ 11.14万
• 依托单位: RESEARCH INSTITUTE FOR MICROBIAL DISEASES, OSAKA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59380005/
• 关键词: Epidemic Hemorrhagic Fever; Hemorrhagic Fever with Renal Syndrome; HFRS Virus; Laboratory Animals; ラット

Two cases of laboratory type HFRS (EHF) were recorded in 1984 in Japan. Thereafter, we have no new patient reported. Every year, we have written annual report of our group-research and the guide-lines for protection and diagnosis of laboratory type infection of HFRS issued by the Ministry of Education, Science and Culture, Japan. We delivered them to all biomedical institutions in Japan. These measures increased the level of understanding of HFRS by biomedical researchers and people working in the animal facilities. As the results, the number of HFRS patients decreased. Most of the cases of the HFRS reported in Japan were caused by viral infection from contaminated laboratory rats. Newborn mice and newborn rats were experimentally infected with viral strains isolated from rats and stripe field mouse. Comparison of the mortality among the infected animals and the recovery of the infective viruses from various organs of them indicates that the animals are more susceptible to the virus st … More rains derived from the animals lelonging to the same genus than those from the animals belonging to the different genus. HFRS viruses are classified as a Bunya virus and devided into four subtypes according to animal species from which they were isolated. Immunological difference of these subtypes was found by using the specific monoclonal antibodies prepared against each viral strain. Electronmicroscopy demonstrated that the HFRS viral particle had a lattice-like surface structure, and some viral particles of certain strains derived from rats had a tail-like structure. Virus strains derived from rats could infect adult rats, resulting in persistnce in several organs. Persistence of the virus in the brain of the infected rats was observed for more than eight months. On the basis of the pathological observation that the virus sometimes caused inflamation of blood vessels, the HFRS might be classified as a "Blood Vessel Disease." Clinical and serological examinations of a HFRS patient was carried out during the hospitalization period. These examinations provided valuable informations for clinical diagnosis. Studies on the low-titre seropositive aniamls and the modes of transmission among experimental animals are to be carried out. Less

1984年在日本记录了两例实验室型肾综合征出血热(EHF)。此后，我们没有新的患者报告。每年，我们都会撰写本课题组的年度报告--日本文部科学省发布的《肾综合征出血热实验室型感染的预防和诊断指南》。我们把它们送到了日本所有的生物医学机构。这些措施提高了生物医学研究人员和动物设施工作人员对肾综合征出血热的了解水平。结果，肾综合征出血热患者数量有所下降。日本报告的大多数肾综合征出血热病例是由受污染的实验室老鼠的病毒感染引起的。用从大鼠和条纹田鼠分离的病毒株感染新生小鼠和新生大鼠。比较感染动物的死亡率和从不同器官中感染病毒的恢复情况，表明动物对st…病毒更敏感。来自同一属的动物的雨量比来自不同属的动物的雨量多。肾综合征出血热病毒被归类为本亚病毒，并根据分离到的动物种类分为四个亚型。用制备的针对各毒株的特异性单抗比较各亚型的免疫学差异。电子显微镜观察表明，HFRS病毒颗粒表面呈点阵状结构，部分鼠源病毒颗粒呈尾状结构。鼠源病毒株可感染成年大鼠，可在多个器官内持续存在。观察了病毒在感染大鼠脑内的持续时间超过8个月。根据病毒有时会引起血管炎症的病理观察，肾综合征出血热可能被归类为“血管疾病”。住院期间对一名肾综合征出血热患者进行了临床和血清学检查。这些检查为临床诊断提供了有价值的信息。将开展低滴度血清阳性疫苗的研究和实验动物之间的传播方式。较少

项目成果

YAMANOUCHI,T.ET AL.,: MICROBIOLOGY & IMMUNOLOGY. 28. 1345-1353 (1984)

山之内 T. 等人：微生物学

森本靖彦: 感染症学雑誌. 60. 322-335 (1986)

森本泰彦：传染病杂志。60. 322-335 (1986)

有川二郎: ウイルス. 36. 233-251 (1986)

有川二郎：病毒。36。233-251（1986）

YAMANISHI, KOICHI, ET AL.,: "ANTIGENIC DIFFERENCES BETWEEN TWO VIRUSES, ISOLATED IN JAPAN AND KOREA, THAT CAUSE HEMORRHAGIC FEVER WITH RENAL SYNDROME" JOURNAL OF VIROLOGY. 52. 231-237 (1984)

YAMANISHI, KOICHI, 等人，：“在日本和韩国分离的两种病毒之间的抗原差异，导致肾综合征出血热”病毒学杂志。

HAMADA, CHUYA, ET AL.,: "AN ATTEMPT TO IMPROVE THE IMMUNOFLUORECENT ANTIBODY TECHNIQUE FOR HEMORRHAGIC FEVER WITH RENAL SYNDROME" EXPERIMENTAL ANIMALS. 35. 1-10 (1986)

Hamada、Chuya 等人：“改进肾综合征出血热免疫荧光抗体技术的尝试”实验动物。