Development of a new cancer therapy using photoexcited semiconductor particles

使用光激发半导体颗粒开发新的癌症疗法

• 批准号: 01870069
• 负责人: KUBOTA Yoshinobu
• 金额: $ 3.39万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01870069/
• 关键词: Photo-chemical reaction; Photoexcited Ti02; Human cancer cells; Semi-conductor Particles; Human Bladder cancer; Cancer treatment; ヒト膀胱癌; 細胞膜

Photo-excited Ti02 semiconductor particles can drive various chemical reactions due to their strong oxidizing and reducing ability. To investigate the possible use of this effect for cancer treatment, the anti-tumor activity of photo-excited Ti02 particles were studied in vitro and in vivo. Human cancer cells, like T-24 human bladder cancer cells and HeLa cells, cultured in vitro were completely killed in the presence of Ti02 with photo-irradiation by a 500 W Hg lamp. In cotrast, very little cell death was observed by Ti02 treatment without photo-irradiation. Photoexcited Ti02 particles also suppressed growth of human cancer cells implanted in nude mice significantly, compared with those receiving Ti02 alone or photo-irradiation alone. The cell death caused by photo-excited Ti02 particles was significantly protected in the presence of L-tryptophan and catalase. These molecules are quenchers of hydroxyl radicals(OH^.) and scavengers of hydrogen peroxide (H_2O_2), respectively, suggesting that the cells were killed by the OH^. and H_2O_2 produced from photo-excited Ti02 particles. On the basis of these experimental observation, it can concluded that the cell killing effect of Ti02 under UV light irradiation could be adopted as one of anti-cancer modality.

光激发的Ti 〇 2半导体颗粒由于其强氧化和还原能力而可以驱动各种化学反应。为了研究这种效应用于癌症治疗的可能用途，在体外和体内研究了光激发的TiO 2颗粒的抗肿瘤活性。体外培养的人癌细胞，如T-24人膀胱癌细胞和HeLa细胞，在Ti 〇 2存在下用500 W Hg灯的光照射完全杀死。在对照中，在没有光照射的情况下，通过Ti 〇 2处理观察到非常少的细胞死亡。与仅接受Ti 〇 2或仅接受光照射的那些相比，光激发的Ti 〇 2颗粒还显著抑制植入裸鼠中的人癌细胞的生长。在L-色氨酸和过氧化氢酶的存在下，由光激发的TiO 2颗粒引起的细胞死亡得到显著保护。这些分子是羟基自由基（OH 2）的猝灭剂。和过氧化氢（H_2O_2）清除剂，表明OH ~（2+）对细胞有杀伤作用。和由光激发的TiO 2颗粒产生的H_2O_2。基于这些实验观察，可以得出结论，TiO 2在紫外光照射下的细胞杀伤作用可以作为抗癌模式之一。

项目成果

Cai.R.,Sakai,H.Hashimoto,K.,Kubota,Y.,Fujishima,A.: "Phagocytosis of titanium dioxide particles and titanium dioxide particles chemically modified by hematoporphylin" Denki Kagaku. 60. 314-321 (1991)

Cai.R.，Sakai，H.Hashimoto，K.，Kubota，Y.，Fujishima，A.：“二氧化钛颗粒的吞噬作用和血卟啉化学修饰的二氧化钛颗粒”电气化学。

R.Cai,Hashimoto,K,K.Ito,Y.Kubota,A.Fujishima: "Photo Killing of malignant Cells with TiO_2 powder" Bull,Chem,Soc,Jpan,. 64. 1268-1273 (1991)

R.Cai，Hashimoto，K，K.Ito，Y.Kubota，A.Fujishima：“用 TiO_2 粉末光杀死恶性细胞” Bull，Chem，Soc，Jpan，。

Kuwamitsu.O.,Kubota.Y.et al.: "Intraarterial chemotherapy combined with surglry for liver metastasis of renal cell carcinoma." Regional Cancer Trentment. 3. 166-168 (1990)

Kuwamitsu.O.，Kubota.Y.等：“动脉内化疗联合手术治疗肾细胞癌肝转移”。

Cai,R.,Hashimoto,K,Kubota,Y,Fujishima A: "Increment of photocatalytic killing of cancer cells using TiO_2 with the and of superoxide dismutase" Chemistry Letter. 1992. 427-430 (1992)

Cai,R.,Hashimoto,K,Kubota,Y,Fujishima A：“使用 TiO_2 和超氧化物歧化酶增强光催化杀伤癌细胞”化学快报。

Cai,R.,Hashimoto,K,Ito,K,Kubota,Y,Fujishima,A,: "Photokilling of malignant cells with TiO_2 powder" Bull,Chem,SoC,Japn.64. 1268-1273 (1991)

Cai,R.,Hashimoto,K,Ito,K,Kubota,Y,Fujishima,A,：“TiO_2 粉末光杀伤恶性细胞”Bull,Chem,SoC,Japn.64。