Analysis of cell polarity associated molecules in prostate cancer for the application to diagnosis and treatment

细胞极性相关分子在前列腺癌尤其是激素难治性前列腺癌中的作用尚不清楚。aPKC是细胞极性的关键分子，在维持细胞分化、生长和极性等方面具有多种作用。因此，我们研究了aPKC在前列腺癌中的表达和功能。aPKC在人前列腺癌组织中的表达水平高于配对的正常前列腺组织。aPKC表达与无复发生存期有关。然后，我们使用siRNA对aPKC进行实验。用siRNA转染的前列腺癌细胞在体外和体内减少了它们的生长。IL-6，其已被称为前列腺癌进展的细胞因子，在siRNA转染的细胞中减少。这些结果表明，aPKC可能通过IL-6的表达在前列腺癌的进展中发挥作用，并且还表明我们先前研究并显示的前列腺癌中aPKC细胞极性系统与局部肾素血管紧张素系统（RAS）之间的密切关系。

The role of cell polarity associated molecules in prostate cancer especially in hormone refractory prostate cancer has been unknown. aPKC which is a key molecule of cell polarity has the various roles for the cell maintenance such as differentiation, growth, and polarity. Thus, we investigated aPKC expression and function in prostate cancer.aPKC expression showed higher levels in human prostate cancer tissues than in paired normal prostate tissues. Moreover, aPKC expression was related to recurrence free survival time. Then, we performed the experiments using siRNA for aPKC. Prostate cancer cells transfected with siRNA reduced their growth in vitro and in vivo. IL-6, which has been known as the cytokine for prostate cancer progression, was reduced in siRNA transfected cells. These results suggest that aPKC may play a role in prostate cancer progression through IL-6 expression, and also are suggested the close relationships between aPKC cell polarity system and local Rennin Angiotensin System (RAS) in prostate cancer which we have studied and have shown previously.