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Application of photoexcited TiO2 semicondoctor to the treatment of bladder cancer

光激发TiO2半导体在膀胱癌治疗中的应用

基本信息

批准号：
05557071
负责人：
KUBOTA Yoshinobu
金额：
$ 3.97万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557071/
关键词：
photoexcited semiconductor TiO2 Bladder cancer 光半導体癌法

项目摘要

A photoexcited titanium dioxide surface has a strong ability to decompose water into hydrogen and oxygen. We have studied this effect in order to use it to kill cancer cells in vitro and in vivo. A distinct cell killing effect was observed on cultured T-24 human bladder cancer cells treated with titanium dioxide particles and 300-400 nm UV light irradiation. Titanium dioxide plus UV light also dramatically suppressed the tumour growth of T-24 cells that were implanted in nude mice. Cells cultured on the titanium dioxide electrode were also killed under UV irradiation when the electrode was anodically polarised, suggesting that photogenerated holes are involved in the cell killing. The cell killing effect caused by titanium dioxide particles plus UV light irradiation was significantly hampered in the presence of L-cysteine and catalase, scavengers of hydroxyl radicals and hydrogen peroxide respectively. Transmission electron microscopic observations showed the titanium dioxide particles to be distributed on the cell surface and inside the cells. These results suggest that titanium dioxide particles under UV light irradiation produced photogenerated holes on the surface yielding hydroxyl radicals and hydrogen peroxide inside or outside the cells and the cells were then killed by the action of these highly oxidising molecules. The possible application of photoexcited titanium dioxide particles to cancer treatment as a new anti-cancer modality is discussed.

光激发的二氧化钛表面具有很强的将水分解成氢和氧的能力。我们已经研究了这种作用，以便在体内和体外使用它来杀死癌细胞。二氧化钛颗粒在300-400 nm紫外光照射下对体外培养的T-24人膀胱癌细胞有明显的杀伤作用。二氧化钛加紫外光也显著抑制了裸鼠体内T-24细胞的肿瘤生长。当电极阳极极化时，在二氧化钛电极上培养的细胞在紫外线照射下也被杀死，这表明光产生的孔参与了细胞的杀死。当羟基自由基清除剂l -半胱氨酸和过氧化氢酶存在时，二氧化钛颗粒加紫外光照射对细胞的杀伤作用明显减弱。透射电镜观察显示，二氧化钛颗粒分布在细胞表面和细胞内部。这些结果表明，在紫外线照射下，二氧化钛颗粒在细胞表面产生光生孔，在细胞内外产生羟基自由基和过氧化氢，然后细胞被这些高度氧化分子的作用杀死。讨论了光激发二氧化钛粒子作为一种新的抗癌方式在癌症治疗中的可能应用。