Study of the identification of new genes inducing hormone refractory prostate cancer

在本研究中，我们尝试用差异显示PCR方法鉴定与激素难治性前列腺癌相关的新的人类基因。对人前列腺癌和正常前列腺组织的RNA进行差异表达基因分析。我们克隆了一百多种可能的基因。和OSM - ?其中nmt55基因为重要基因。OSM-β基因是酪氨酸激酶型受体家族基因之一。该基因在激素难治性前列腺癌中高表达，可能与雄激素非依赖性生长特性有关。nmt55基因被编码为转录因子之一。我们已经证明该基因的表达与雄激素受体的表达密切相关。该基因在激素难治性前列腺癌中最后消失，提示与雄激素不依赖型生长调节有关。我们还分析了LNCaP人前列腺癌细胞经双氢睾酮处理后的RNA差异表达基因。两个重要的基因？克隆了2和NKx 3.1样基因。雄激素可下调利素α-2基因的表达，该基因可能与雄激素剥夺后诱导细胞凋亡有关。由于lipin α-2基因在雄激素非依赖型前列腺癌细胞系（PC-3、DU-145）或激素难愈性前列腺癌组织中表达完全消失，提示该基因在诱导激素非依赖型前列腺细胞中发挥作用。

In this research, new human genes associated with hormone refractory prostate cancer were tried to be identified by applying differential display PCR methodology. The RNA of human Prostate cancer and normal prostate tissue was analyzed for differentially expressed gene. We have clones more than one hundred possible genes. And OSM-? and nmt55 genes were identified as important genes.OSM-β gene is one of the tyrosine kinase type receptor family genes. This gene was shown to be highly expressed in hormone refractory prostate cancer and suggested to be involves in androgen independent growth characteristics.The nmt55 gene is coded as one of the transcriptional factors. We have shown that the expression of this gene was closely associated with the expression of androgen receptor. This gene was shown to be disappear last in hormone refractory prostate cancer and suggested to be associated with androgen independent growth regulation.We have also analyzed the RNA of LNCaP human prostate cancer cells treated with dihydrotestosterone for differentially expressed gene. Two important genes Liprin?-2 and NKx 3.1 like gene were cloned.Liprinα-2 gene expression was shown to be down-regulated by androgen and this gene might be associated with the induction of apoptosis after androgen deprivation. Since Liprinα-2 gene expression disappeared completely lost in androgen independent prostate cancer cell line (PC-3, DU-145) or hormone refractory prostate cancer tissues, this gene is suggested to play a roll in the induction of hormone independent prostate cells.