Application of High-Performance Liquid Chromatography with A New Photometric Detection Method (Development of Analytical Methods for Environmental Pollutants)

新型光度检测方法高效液相色谱的应用（环境污染物分析方法的发展）

基本信息

批准号：
02670975
负责人：
HAYAKAWA Kazuichi
金额：
$ 1.41万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

(1) Theoretical studies on the retention and peak formation mechanism in ion chromatography (IC) : Following on from our previous work, a new theory "selective peak enhancement and suppression of conductivity detection IC" was evaluated. This theory was effectively used in the determination of trace level orthophosphate in sewage samples. Ion exchange chromatographic behavior of polyvalent eluent systems was evaluated theoretically by introducing a new parameter "the intereluent separation factor" in equations for Hoover's model.(2) Functional eluents for photometric detection IC : Both[Cu(en)_2SO_4 and[Cu(trien)]SO_4 were effective eluents for the sensitive determination of cations. Using a Na_2[Cu(edta)]- Na_2H_2 edta eluent, not only anions but also metal ions were deterifined as metal-edta anion chelates by photometric detection IC. These methods were effectively used in the determination of cations and anions in environmental water samples. Malic acid enantiomers were separately d … More etermined by photometric detection IC using a copper - tartaric acid complex eluent, based on a ligand-exchange formation mechanism. Malic acid, an apple juice additive, was determined by this method.(3) Determination of carbonate-carbon : Interfering atmospheric carbon dioxide dissolved in eluents was removed effectively by nitrogen purging. Using this equipment, trace level carbonate carbon as low as 10^<-6> M could be determined by photomeric detection IC as well as ion exclusion chromatography (conductivity detection). Carbonate-carbon in rain water was determined by this method.(4) Polarized photometric detection : When two polarizers were set on both sides of the flow sell of an absorbance detector, the intensity of natural light transmitted through the polarizers and the flow cell was changed according to the optically active compounds if passed through. The change in absorbance was in proportion to the concentration of the compound. Nonchromophoric optically active compounds such as sugars were sensitively determined using photometric detection HPLC without any derivatization.(5) Automatic pretreatment/HPLC system : The combination of column-switching system and a heart-cutting technique effectively removed interfering compounds in IC. This system was used to determine anions in river and rain water samples by photometric detection IC and orthophosphate in sewage samples by conductivity detection IC.(6) Determination of nitopyrenes by HPLC with chemiluminescence detection : A method of determining nitro-, nitroso- and aminopyrenes has been developed using HPLC with chemiluminescence detection after on-line electrochemical reduction. Determination method of 1, 3-, 1, 6-, 1, 8-dinitropyrenes and 1-nitropyrene has also been developed using HPLC with chemiluminescence detection after off-line chemical reduction. Utilizing these methods, dinitropyrenes and nitropyrene were determined in diesel- and gasoline-engine exhaust particulates. Less

（1）关于离子色谱（IC）中保留和峰形成机理的理论研究：从我们先前的工作中，评估了一种新的理论“选择性峰值增强和抑制电导率检测IC”。该理论有效地用于确定污水样品中的痕量高磷酸盐。 Ion exchange chromatographic behavior of polyvalent eluent systems was evaluated theoretical by introducing a new parameter "the intereluent separation factor" in equations for Hoover's model.(2) Functional elastics for photometric detection IC: Both[Cu(en)_2SO_4 and[Cu(trien)]SO_4 were effective elastics for the sensitive determination of cations.使用Na_2 [Cu（EDTA）] -na_2h_2 EDTA弹性，不仅阴离子，而且金属离子也被确定为金属-EDTA阴离子樱桃，通过光度检测IC确定。这些方法有效地用于测定环境水苹果元中的阳离子和阴离子，分别使用了基于配体换剂形成机制的铜 - tartaric酸复合物弹性，可以通过光度检测IC进行光度检测IC。苹果汁添加剂是通过这种方法确定的。（3）确定碳酸盐 - 碳酸盐：干扰溶解在弹性中的大气二氧化碳通过氮气清除有效去除。使用此设备，可以通过光组检测IC以及离子排除色谱法（电导率检测）确定痕量碳酸盐碳的低至10^<-6> m。雨水中的碳酸盐碳是通过这种方法确定的。（4）极化光度检测：当在吸光度检测器的流量销售的两侧设置两个极化器时，如果通过偏振器传播的自然光强度，则根据光学电池的变化，如果通过光学活性化合物。吸收的变化与化合物的浓度成正比。使用光度检测HPLC敏感地确定非鸡蛋光活性化合物，而无需任何衍生化。（5）自动预处理/HPLC系统：柱转换系统和心脏切割技术的组合有效地除去了IC中的干扰化合物。该系统用于通过电导率检测IC中的光度检测IC和正磷酸盐来确定河流和雨水样品中的阴离子。（6）通过化学发光检测测定HPLC的硝基py虫：一种确定硝基 - 氮气，氮气和杏仁氏菌的方法，使用Hplc Replectro in Chemiminals plincection开发了硝基 - ，氮气和杏仁症的方法。在离线化学还原后，使用HPLC进行了化学发光检测，也已经开发了1、3-、1、6-，1、8-二硝基副本和1-硝基py的测定方法。利用这些方法，在柴油机和汽油引擎排气组件中确定了二硝基副本和硝基吡啶。较少的