Genetic Study of Nitrate Transport across the Plasma Membrane of Root Epidermal Cells in Some Crop Plants
某些作物根表皮细胞质膜硝酸盐转运的遗传研究
基本信息
- 批准号:03660007
- 负责人:
- 金额:$ 1.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Breeding of crops with efficient use of plant nutrient is one of the goals for low-input crop production. In this study, two kinetic parameters, Km and Vmax, for high affinity nitrate uptake by young seedlings were determined in several crop plants for evaluating the expression of nitrate uptake gene (s). Summary of the results are shown as follows.1) In twenty Japanese barley cultivars, Km and Vmax were 50.9-111.7 mu M and 2.24-4.99 mu mol/g/h, respectively. IIowever, these results were thought to be underestimated because of experimental procedure. 2) In barley genotypes with different water tolerance at seedling stage, Km and Vmax were 143.0-209.4 mu M and 3.37-7.85 mu mol/g/h, respectively. No relationships between the kinetic parameters and water tolerance was observed. 3) In sixteen rice cultivars including both indica and japonica, Km and Vmax were 75.7-130.3 mu M and 4.30-7.17 mu mol/g/h, respectively. In the cultivars of indica and japonica showing similar level of Km, indica cultivars tended to show lower Vmax than japonica cultivars. 4) Km and Vmax in several genotypes of wheat, rye and Triticale were similar level of those of barley and rice. 5) In four rice mutants affecting nitrate assimilation, M605, a chlorate hypersensitive mutant, showed significantly higher Km than that of the wild type, Norin 8. Furthermore, there was difference in ratio of nitrate uptake ability from 40 mu M KNO_3 to that from 400 mu M KNO_3 in 145 rice genotypes, suggesting variability in the kinetic parameters.
培育高效利用植物养分的作物是低投入作物生产的目标之一。本研究测定了几种作物幼苗高亲和性硝酸盐吸收的动力学参数Km和Vmax,以评价硝酸盐吸收基因的表达。结果表明:(1)20个日本大麦品种的Km和Vmax分别为50.9-111.7 μ M和2.24-4.99 μ mol/g/h。然而,这些结果被认为是低估,因为实验程序。2)苗期不同耐水性大麦基因型的Km和Vmax分别为143.0-209.4 μ M和3.37-7.85 μ mol/g/h。动力学参数和耐水性之间没有关系。3)16个水稻品种(包括籼、粳稻)的Km和Vmax分别为75.7-130.3 μ M和4.30-7.17 μ mol/g/h。在Km值相近的籼粳品种中,籼型品种的Vmax值往往低于粳型品种。4)小麦、黑麦和小黑麦的Km和Vmax与大麦和水稻相近。5)在4个影响水稻硝酸盐同化的突变体中,氯酸盐敏感突变体M605的Km值显著高于野生型Norin 8。此外,145个水稻基因型对40 μ M KNO 3和400 μ M KNO 3的硝酸盐吸收能力之比存在差异,表明动力学参数存在变异性。
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
森田和恵・長谷川博・中村明夫: "タバコ葯培養に及ぼすガンマ線の効果" 近畿作物育種研究. 38. 61-64 (1993)
Kazue Morita、Hiroshi Hasekawa 和 Akio Nakamura:“伽马射线对烟草花药培养的影响”近畿作物育种研究 38. 61-64 (1993)。
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長谷川博: "わが国オオムギ品種における硝酸イオン吸収のミハエリス=メンテン式による解析." 日本作物学会紀事. 61. 251-256 (1992)
Hiroshi Hasekawa:“使用 Michaelis-Menten 方程分析日本大麦品种的硝酸根离子吸收。”日本作物科学学会杂志 61. 251-256 (1992)。
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Masahiko ICHll,Toyomasa KATAGIRI and Hiroshi HAEGAWA: "Mutants with low nitrate reductase activity selected from seedlings expressing nitrogen deficiency symptoms in rice coryza sativa〓‐)" Japanese Journal of Breeding. 43. 123-127 (1993)
Masahiko ICHll、Toyomasa KATAGIRI 和 Hiroshi HAEGAWA:“从表达水稻缺氮症状的幼苗中选择的具有低硝酸还原酶活性的突变体〓-)”日本育种杂志 43. 123-127 (1993)。
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長谷川 博・川崎 俊己 市橋 秀樹・渡辺 洋: "数種作物の根における硝酸イオンキャリアーの特性.ミハエリス=メンテン定数および誘導時間について." 近畿作物・育種研究. 37. 95-101 (1992)
Hiroshi Hasekawa、Toshiki Kawasaki、Hideki Ichihashi 和 Hiroshi Watanabe:“几种作物根部硝酸根离子载体的特性。关于米氏常数和诱导时间。近畿作物育种研究”。
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- 影响因子:0
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中村明夫・高島 賢・長谷川 博・松本 豪: "タバコ半数体幼苗のコルヒチン処理による簡易で効率的な染色体倍加法" 育種学雑誌. 43. 603-612 (1993)
Akio Nakamura、Satoshi Takashima、Hiroshi Hasekawa 和 Go Matsumoto:“使用秋水仙碱处理单倍体烟草幼苗的简单有效的染色体加倍方法”《育种科学杂志》43. 603-612 (1993)。
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HASEGAWA Hiroshi其他文献
発展的思考・態度の育成を指向した授業評価ルーブリックの検討-観点「子供の反応を知る」の基準-
审查以培养发展性思维和态度为导向的课程评价标准 - “了解儿童反应”视角的标准 -
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
KITPO Nuttakarn;KONGWAT Suphanut;HIRABAYASHI Takara;LAWANONT Worawat;HASEGAWA Hiroshi;INOUE Masahiro;KHANTACHAWANA Anak;菊池聡;大南樹生,伊東良晴,中釜達朗;加藤栞里・桐生徹・大島崇行;重松敬一 - 通讯作者:
重松敬一
教室内実験を志向した教育用可搬型Green HPLCシステムの試作
用于课堂实验的教育用途便携式绿色 HPLC 系统原型
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
KITPO Nuttakarn;KONGWAT Suphanut;HIRABAYASHI Takara;LAWANONT Worawat;HASEGAWA Hiroshi;INOUE Masahiro;KHANTACHAWANA Anak;菊池聡;大南樹生,伊東良晴,中釜達朗 - 通讯作者:
大南樹生,伊東良晴,中釜達朗
Learning Outcomes of Global Project-Based Learning: Insights From Teaching Assistants
全球项目式学习的学习成果:助教的见解
- DOI:
10.4307/jsee.68.5_39 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
KITPO Nuttakarn;KONGWAT Suphanut;HIRABAYASHI Takara;LAWANONT Worawat;HASEGAWA Hiroshi;INOUE Masahiro;KHANTACHAWANA Anak - 通讯作者:
KHANTACHAWANA Anak
HASEGAWA Hiroshi的其他文献
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{{ truncateString('HASEGAWA Hiroshi', 18)}}的其他基金
Development of a multisensory fusion ICT system transmitting attractiveness of traditional crafts
开发传递传统工艺魅力的多感官融合ICT系统
- 批准号:
18H03458 - 财政年份:2018
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A Study on the Long-distance Trades Conducted in The Medieval Western Japan Sea
中世纪西日本海长途贸易研究
- 批准号:
18K00927 - 财政年份:2018
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effect of hyperhomocysteinemia on the cardiovascular disease in renal failure
高同型半胱氨酸血症对肾功能衰竭心血管疾病的影响
- 批准号:
17K08428 - 财政年份:2017
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A Study on the Water Transport in the Western Japan Sea in the Middle Ages and the Distribution Structure of the Sanin Area
中世纪西日本海的水运及山阴地区的分布结构研究
- 批准号:
15K02832 - 财政年份:2015
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Remediation of toxic metal contaminated soils by washing with biodegradable chelants
用可生物降解的螯合剂清洗修复有毒金属污染的土壤
- 批准号:
24310056 - 财政年份:2012
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Reconstruction of space plasma structures in four-dimensional spacetime from multi-spacecraft observations
多航天器观测重建四维时空空间等离子体结构
- 批准号:
24740337 - 财政年份:2012
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Syntactic and Lexical Study of Reflexives Based on Multilingual DVD Captions Database
基于多语言DVD字幕数据库的反身句法和词汇研究
- 批准号:
24520554 - 财政年份:2012
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Evaluation of subjective equality between a visual stimulus and its associated sound in an audio-video space
视听空间中视觉刺激与其相关声音之间的主观平等评估
- 批准号:
24500252 - 财政年份:2012
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A Study of Medieval MUNAFUDA in the San-in Region
山阴地区中世纪MUNAFUDA研究
- 批准号:
24520751 - 财政年份:2012
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional role of brain neurotransmitters on exercise capacity and body temperature regulation
脑神经递质对运动能力和体温调节的功能作用
- 批准号:
23700773 - 财政年份:2011
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
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