Development of foam countercurrent chromatography for application works

近年来，泡沫逆流色谱技术的发展实现了泡沫分离技术的创新，并成功地应用于不含表面活性剂或其他添加剂的氮气和蒸馏水中疏水性组分的分离和浓缩。结果表明，该方法可有效地用于泡沫活性成分的分离和浓缩。为了促进该技术在各种天然产品中的应用，引入一套可靠地表明样品对泡沫CCC适用性的物理化学参数，并开发一种样品注入方法，通过该方法可以将大量的样品溶液引入泡沫CC中。为了简单地测试泡沫CCC对样品的适用性，作者引入了两个参数，即“发泡力”和“泡沫稳定性”。在每一次测试中，样品都被送入有刻度的圆柱形…越呃，紧接着就是剧烈的摇晃。发泡能力由产生的泡沫与剩余溶液的体积比表示，泡沫的稳定性由泡沫的持续时间表示。从杆菌肽、栀子黄、黄素B和玫瑰红等不同的样品中获得的一组数据表明，这些参数与泡沫CC对泡沫的富集度之间存在显著的相关性。总体结果表明，泡沫CC可以有效地对具有以下特性的样品进行富集化：发泡力大于1.0，泡沫稳定性大于250分钟。在泡沫CC中引入大量杆菌肽溶液，证明了该体系的实用性。从原始样品溶液中几乎分离出疏水成分杆菌毒素A，并在泡沫组分中进行了浓缩。因此，该循环进样系统能较好地分离和浓缩杆菌毒素复合体中的疏水组分。该方法对于从天然粗产物中分离和浓缩化合物是非常有效的。较少

Recently, an innovation of the foam separation technology has been achieved by development of foam countercurrent chromatography (Foam CCC), which has been successfully applied to separation and enrichment of hydrophobic components in a sample using nitrogen gas and distillled water free of surfactant or other additives. The results indicated that the method may be effectively applied to separation and enrichment of foam active components. In order to facilitate the application of the technique to various natural products, it is highly desirable to introduce a set of physicochemical parameters which reliable indicate applicability of a sample to the foam CCC and to develop a sample injection method, by which a large volume of the sample solution can be introduced into the foam CCC.To simply test the applicability of foam CCC to a sample the authors have introduced two parameters, i. e., "foaming power" and "foam stability". In each test, the sample was delivered into a graduated cylind … More er followed by vigorous shaking. The foaming power was expressed by the volume ratio of the resulting foam to the remaining solution, and the foam stability by the duration of the foam. A set of data obtained from various samples including bacitracin, gardenia yellow, phloxine B, and rose bengale revealed a significant correlation between these paramenters and the degree of foam enrichment by foam CCC.The overall results indicated that the sample with the following properties may be effectively enriched by foam CCC : the foaming power is greater than 1.0 and the foam stability is over 250 min.For the sample injection method, a new system named "recycle injection system" has been developed. The utility of this system was demonstrated in the introduction of a large volume of bacitracin solution to the foam CCC.A hydrophobic component, bacitracin A, has been almost isolated and enriched in the foam fraction from the original sample solution. Thus, satisfactory separation and enrichment of the hydrophobic components of bacitracin complex could be obtained by this recycle injection system. The method would be very effective for separation and enrichment of a compound from crude natural products. Less