The macrophage nucleus - its form and function during migration in vivo

巨噬细胞核 - 在体内迁移过程中的形式和功能

• 批准号: DP210103263
• 负责人: Prof Graham Lieschke
• 金额: $ 27.44万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-08-04 至 2024-08-03
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210103263
• 关键词: macrophage; nucleus; its; form; function

As cells migrate through tissues, they encounter complex, 3-dimensional environments that provide cues to guide them and present obstacles in their path. This project focuses on macrophages, a large immune cell capable of both amoeboid and mesenchymal modes of migration. The nucleus is the largest organelle and its bulk and stiffness must be managed as migrating cells travel through constrictions. The project uses specialised high-end microscopy and genetic methods to examine how the nucleus of migrating zebrafish macrophages deforms, repositions and is restructured during migration in living tissues, and how this influences macrophage locomotion. The goal is to provide fundamental insights into the cell biology of macrophage migration.

当细胞在组织中迁移时，它们会遇到复杂的三维环境，这些环境为它们提供引导线索，并在它们的路径上设置障碍。该项目重点研究巨噬细胞，巨噬细胞是一种能够以变形虫和间充质方式迁移的大型免疫细胞。细胞核是最大的细胞器，它的体积和硬度必须在迁移细胞通过收缩时进行管理。该项目使用专业的高端显微镜和遗传方法来研究迁移斑马鱼巨噬细胞的细胞核在活组织迁移过程中如何变形、重新定位和重组，以及这如何影响巨噬细胞的运动。目的是为巨噬细胞迁移的细胞生物学提供基本的见解。