Linking mutant zebrafish phenotypes with their underlying genetic lesions.

斑马鱼突变体已经产生了许多有趣的异常，但要了解这些异常，必须通过定位克隆鉴定缺陷基因。我们试图找出导致四种突变的缺陷基因。突变体#562发育成一个正常的神经系统，然后经历快速退化。突变的小花洛特有不正常的肠道发育。将研究两种早期血液形成缺陷的突变体。我们将建立对所有斑马鱼测绘项目有用的几个步骤的技术。我们期望这些突变体的遗传特征为神经细胞存活、肠道发育和血液形成提供新的见解。

Zebrafish mutants have been generated with many interesting abnormalities, but to understand these abnormalities, the defective genes must be identified by positional cloning. We seek to identify the defective genes underpinning four mutants. Mutant #562 develops a normal nervous system which then undergoes rapid degeneration. The mutant flotte lotte has abnormal gut development. Two mutants with defective early blood formation will be studied. We will establish techniques for several steps that will be useful for all zebrafish mapping projects. We expect the genetic characterization of these mutants to provide new insights into nerve cell survival, gut development, and blood formation.