Linking mutant zebrafish phenotypes with their underlying genetic lesions.

将突变斑马鱼表型与其潜在的遗传病变联系起来。

• 批准号: DP0346823
• 负责人: Prof Graham Lieschke
• 金额: $ 7.73万
• 依托单位: Ludwig Institute for Cancer Research Ltd
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0346823
• 关键词: Linking; mutant; zebrafish; phenotypes; their

Zebrafish mutants have been generated with many interesting abnormalities, but to understand these abnormalities, the defective genes must be identified by positional cloning. We seek to identify the defective genes underpinning four mutants. Mutant #562 develops a normal nervous system which then undergoes rapid degeneration. The mutant flotte lotte has abnormal gut development. Two mutants with defective early blood formation will be studied. We will establish techniques for several steps that will be useful for all zebrafish mapping projects. We expect the genetic characterization of these mutants to provide new insights into nerve cell survival, gut development, and blood formation.

斑马鱼突变体已经产生了许多有趣的异常，但要了解这些异常，缺陷基因必须通过定位克隆。我们试图找出支持四个突变体的缺陷基因。突变体#562发育出一个正常的神经系统，然后迅速退化。突变的flotte乐天有异常的肠道发育。将研究两种具有缺陷的早期血液形成的突变体。我们将建立几个步骤的技术，这将是有用的所有斑马鱼绘图项目。我们期望这些突变体的遗传特征能够为神经细胞存活、肠道发育和血液形成提供新的见解。