Nuclear plasticity during neutrophil migration and function

中性粒细胞迁移和功能过程中的核可塑性

• 批准号: DP170102235
• 负责人: Prof Graham Lieschke
• 金额: $ 27.3万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2020-09-11
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170102235
• 关键词: Nuclear; plasticity; during; neutrophil; migration

This project aims to discover how nuclear shape affects neutrophil function. Cell migration needs overall cellular plasticity and plasticity of internal structures such as the nucleus. The neutrophil, one of the most peripatetic cell types, has a specialised lobulated nucleus, thought to facilitate its mobility and function. Using zebrafish reporter lines that concurrently display the nucleus and cytoplasm, this project will display the dynamic plasticity of neutrophil nuclei during neutrophil migration and function in vivo. This project seeks to use the spatiotemporal resolution of a lattice light sheet microscope to examine this further, and explore its effect on neutrophil function. The project seeks to establish morphological and mechanical principles applying not just to neutrophils, but to all migratory cell types.

该项目旨在发现核形状如何影响中性粒细胞功能。细胞迁移需要细胞整体的可塑性和细胞核等内部结构的可塑性。中性粒细胞是最具活动性的细胞类型之一，具有专门的分叶状核，被认为有助于其移动和功能。利用同时显示细胞核和细胞质的斑马鱼报告细胞系，该项目将显示中性粒细胞迁移和功能过程中中性粒细胞核的动态可塑性。本项目旨在使用点阵光片显微镜的时空分辨率来进一步研究这一点，并探索其对中性粒细胞功能的影响。该项目旨在建立形态学和机械原理，不仅适用于中性粒细胞，而且适用于所有迁移细胞类型。