Structural elucidation of isolated and fractionated lignins by means of ultra-high resolving mass spectrometry (LignoMassStructure)

通过超高分辨率质谱分析分离和分馏木质素的结构 (LignoMassStructure)

• 批准号: 525499342
• 负责人: Professor Dr. Steffen Fischer
• 金额: --
• 依托单位: Institut für Analytische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/525499342?language=en
• 关键词: Structural; elucidation; isolated; fractionated; lignins

The goal of this research project is a development of ESI- and GALDI-FT-ICR-MS and tandem MS routines for a characterization of isolated and fractionated lignins from different plants (coniferous wood, deciduous wood and annual plants) and pulping processes (kraft, sulfite and soda pulping). These routines will massively help to reduce the effort for an analysis of soluble and insoluble lignin samples, in comparison to classical analytical methods for a lignin characterization (e.g., GPC, NMR, Py-GC/MS). On the one hand, general sample information are accessible with these FT-ICR-MS and -MS/MS methods, such as molar mass distribution. On the other hand, also specific structural insights into the lignin samples are obtainable by these experiments. For this purpose, method development and optimization experiments will be performed with the help of a fractionated kraft lignin from deciduous wood (beech). In addition, a hetero spectroscopic correlation approach will be applied to combine the FT-ICR-MS and -MS/MS results with results from other analytical techniques (Py-GC/MS, 13C-, 31P- and 2D-HSQC-NMR spectroscopy), which will help to obtain more information about the structural composition of the different lignins. These novel results will significantly increase our understanding of the influence of an isolation process on the lignin target structure. Especially the GALDI-FT-ICR-MS and -MS/MS analyses of solid lignin samples will be helpful to characterize the lignin structure more detailed, independently from the solubility of the lignin compounds contained in the different samples or sample fractions. In the last project phase, the capabilities of the developed methods will be demonstrated by analyzing a complex lignocellulose, which will help to evaluate the applicability of the developed routines for other biopolymers (cellulose and hemicellulose). If this method application step is successful, for the first time ever, a structural characterization of biopolymers in their native environment is possible. Therefore, the results from this research project will have an impact on the development of more effective and expedient isolation and modification procedures for biopolymers, which will significantly contribute to a production of lignin and lignocellulose based products of higher value in the near future.

本研究项目的目标是开发ESI-和Galdi-FT-ICR-MS和串联MS程序，用于表征来自不同植物(针叶木、落叶木和一年生植物)和制浆过程(硫酸盐、亚硫酸盐和纯碱法制浆)的分离和分级木质素。与木质素表征的经典分析方法(例如，GPC、核磁共振、Py-GC/MS)相比，这些常规程序将极大地帮助减少分析可溶和不溶木质素样品的工作量。一方面，通过这些FT-ICR-MS和-MS/MS方法可以获得一般的样品信息，如摩尔质量分布。另一方面，通过这些实验也可以获得对木质素样品的特定结构洞察。为此，将利用从落叶木材(山毛榉)中分离出的硫酸盐木质素进行方法开发和优化实验。此外，还将使用异谱相关方法将FT-ICR-MS和-MS/MS结果与其他分析技术(Py-GC/MS，13C-，31P-和2D-HSQC-NMR谱)的结果相结合，这将有助于获得关于不同木质素结构组成的更多信息。这些新的结果将大大增加我们对分离过程对木质素靶标结构的影响的理解。特别是固体木质素样品的Galdi-FT-ICR-MS和-MS/MS分析将有助于更详细地表征木质素的结构，而不依赖于不同样品或样品组分中木质素化合物的溶解度。在最后的项目阶段，将通过分析复杂的木质纤维素来演示所开发方法的能力，这将有助于评估所开发的常规程序对其他生物聚合物(纤维素和半纤维素)的适用性。如果这一方法应用步骤成功，有史以来第一次，生物聚合物在其自然环境中的结构表征是可能的。因此，这一研究项目的结果将对开发更有效和更方便的生物聚合物分离和改性程序产生影响，这将在不久的将来显著有助于生产更高价值的木质素和木质纤维素产品。