Basic and applicable studies on transgenicfish.

转基因鱼的基础与应用研究。

• 批准号: 05404014
• 负责人: TAKASHIMA Fumio
• 金额: $ 10.3万
• 依托单位: Tokyo University of Fisheries
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05404014/
• 关键词: gene; transgenic; fish; micro injection; electroporation; 三倍体; 成長ホルモン遺伝子

The purpose of this project is to search and analyze the structure of fish genes. At the same time, transgenic effect of foreign gene into fertilized eggs was also examined.(1).We have succeeded to obtain repeatable genes from medaka DNA and the clones of these gene.(2).Subunit structure of multiple hemoglobins in carp was demonstrated.(3).Functional analysis of promoter regions from carp a-globin genes in cultured fish cells was done.(4).The nucleotide sequence of cDNA for yamame salmon growth hormone was shown.(5).Sterile triploidy production in fish (pejerrey) was succeeded. This technique is useful to introduce transgenenic fish without distribution of the gene in the ecosystem.(6).High efficiency of foreign gene introduction was clearly demonstrated by osmotic changes of sperm plasma (extender) at the time electroporation.(7).Dinecleotide-repeat polymorphism in DNA of rainbow trout was recognized.(8).Variable number of tandem repeat markers were demonstrated in rainbow trout.

本项目的目的是寻找和分析鱼类基因的结构。同时，对外源基因转入受精卵的效果也进行了检测。(1).我们成功地从青竹DNA及其克隆中获得了可重复的基因。(2)证明了鲤鱼体内多种血红蛋白的亚基结构。(3)对鲤鱼α-珠蛋白基因启动子区域在养殖鱼类细胞中的功能进行了分析。(4)获得了山鲑鱼生长激素基因的核苷酸序列。(5)成功地在鱼类(PeJerrey)中进行了无性系三倍体生产。这项技术有助于在没有基因在生态系统中分布的情况下引入转基因鱼类。(6).通过电穿孔时精子血浆(延伸物)的渗透变化清楚地证明了外源基因导入的高效性。(7).识别出虹鱼DNA中的二核苷酸重复多态性。(8).在虹鳟鱼中发现了数量不等的串联重复标记。

项目成果

Aoki 他: "Effect of Actiuin A on carp α-glofin gene expression in human K562 cells" I mar,Biotechnol.,. 2. 59-60 (1994)

Aoki 等人：“Actiuin A 对人 K562 细胞中鲤鱼 α-球蛋白基因表达的影响”I mar，Biotechnol.， 2. 59-60 (1994)

M.Nagano.G.Yoshizaki, I.Hirono, T.Oshiro and F.Takashima: "The nucleotide sequence of cDNA for yamame salmon growth hormone." Fisheries Science. 60. 237-238 (1994)

M.Nagano.G.Yoshizaki、I.Hirono、T.Oshiro 和 F.Takashima：“山目鲑鱼生长激素 cDNA 的核苷酸序列。”

C.A.Strussmann, S.Moriyama, E.F.Hanke, J.S.Calsina Cota and F.Takashima: "Evidence of thermolabile sex determination in pejerrey" Odontesthes bonariensis.J.Fish Biol.(in press).

C.A.Strussmann、S.Moriyama、E.F.Hanke、J.S.Calsina Cota 和 F.Takashima：“pejerrey 耐热性别决定的证据”Odontesthes bonariensis.J.Fish Biol.（出版中）。

Okamoto,N.他: "Two c-myc genes from a tetraplord fish,the common carp." Gene. 153. 231-236 (1995)

Okamoto, N. 等人：“来自四足鱼（鲤鱼）的两个 c-myc 基因。” 153. 231-236 (1995)

隆島 他: "Submit structures of multiple hemglobins in carp" J.Comp.Physiol.B. 163. 445-451 (1993)

Takashima 等人：“提交鲤鱼中多种血红蛋白的结构”J.Comp.Physiol.B 163. 445-451 (1993)