Basic and applicable studies on transgenicfish.

本项目的目的是对鱼类基因的结构进行检索和分析。同时，还考察了外源基因对受精卵的转基因效果。我们已经成功地从medaka DNA中获得了可重复的基因和这些基因的克隆。研究了鲤鱼体内多种血红蛋白的亚基结构。对鲤鱼a-珠蛋白基因启动子区域在养殖鱼细胞中的功能进行了分析。显示了山麻鲑鱼生长激素cDNA的核苷酸序列(5)。成功地在鱼（pejerrey）上产生了不育三倍体。该技术有助于在生态系统中不分布基因的情况下引入转基因鱼。电穿孔时精子血浆（扩展剂）的渗透变化清楚地证明了外源基因导入的高效率(7)。发现了虹鳟鱼DNA的二肽重复多态性(8)。在虹鳟鱼中发现了不同数量的串联重复标记。

The purpose of this project is to search and analyze the structure of fish genes. At the same time, transgenic effect of foreign gene into fertilized eggs was also examined.(1).We have succeeded to obtain repeatable genes from medaka DNA and the clones of these gene.(2).Subunit structure of multiple hemoglobins in carp was demonstrated.(3).Functional analysis of promoter regions from carp a-globin genes in cultured fish cells was done.(4).The nucleotide sequence of cDNA for yamame salmon growth hormone was shown.(5).Sterile triploidy production in fish (pejerrey) was succeeded. This technique is useful to introduce transgenenic fish without distribution of the gene in the ecosystem.(6).High efficiency of foreign gene introduction was clearly demonstrated by osmotic changes of sperm plasma (extender) at the time electroporation.(7).Dinecleotide-repeat polymorphism in DNA of rainbow trout was recognized.(8).Variable number of tandem repeat markers were demonstrated in rainbow trout.