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Molecular ecology of plant populations using microsatellite genetic markers

使用微卫星遗传标记的植物种群的分子生态学

基本信息

批准号：
06454010
负责人：
KAWANO Shoichi
金额：
$ 4.86万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454010/
关键词：
Arabidopsis thaliana microsatellite marker plant molecular ecology nolecular population genetics ecotype phenotypic plasticity population differentiations character expression アロザイム DNA メタ個体群ブナアメリカブナ Uvularia マイクロサテライトマーカー

项目摘要

1.Microsatellite polymorphisms of Arabidopsis thaliana were surveyed for 29 loci using 12 Japanese natural populations as material. All the individuals examined were homozygous at all the microsatellite loci. Although there was considerable variation among populations, no intra-population polymorphism was detected in three selected populations when eight loci were studied. The microsatellites examined proved to be a suitable genetic marker for studying the genetic variation among natural populations of A.thaliana. To compare the genetic similairty and infer the evolutionary relationships among populations, neighbor-joining trees were constructed on the basis of distance matrices calculated by two different methods. Genetic relationships among the populations as revealed in the trees do not necessarily correspond to their geographic telation-ships. This may reflect their different origins or different times of introudction into the lslands of Japan.2.Phenotypic plasticity of A.thaliana collected from 11 different wild populations in Japan was experimentally examined under four different conditions : (1) GVLD (green vernalization-long day, 16hrs), (2) GVSD (green vernalization-short day, 8hrs), (3) LD (no vernalization-long day), and (4) SD (no vernalization-short day). 440 plants (10 replicates*4 treatments*11 populations) were included for this experiment. The results indicate that long-day and/or low temperature shortened the days to flowering except Kanagawa, and long-day was more effective than low temperature. The effects of two treatments were additive because the flowering was most accelerated when the long-day and vernalization treatment were applied in combination. Several other correlated patterns of character changes were recorded, reflecting the origin of populations and experimental treatments.

1.以12个日本自然居群为材料，对拟南芥29个位点的微卫星多态性进行了调查。所有个体在所有微卫星位点均为纯合子。虽然群体间存在相当大的变异，但在8个位点的研究中，3个选择的群体未发现群体内多态性。结果表明，微卫星是研究拟南芥自然居群间遗传变异的合适遗传标记。为了比较遗传相似性和推断种群间的进化关系，在两种方法计算的距离矩阵的基础上构建了邻居连接树。这些树所揭示的种群间的遗传关系并不一定与它们的地理关系相符。这可能反映了它们的不同起源或传入日本岛屿的不同时间。采用4种不同条件(1)GVLD（绿春化-长日照，16小时）、(2)GVSD（绿春化-短日照，8小时）、(3)LD（无春化-长日照）和(4)SD（无春化-短日照）对日本11个不同野生种群的拟南芥表型可塑性进行了实验研究。试验选用440株（10个重复*4个处理*11个群体）。结果表明，除神奈川县外，长日照和（或）低温均缩短了花期，且长日照比低温更有效。两种处理的效果是叠加的，因为长日照和春化处理联合施用时开花速度最快。还记录了其他几个相关的性状变化模式，反映了种群的起源和试验处理。