Self-Assembled Molecular Layrs with Biological Functions -Constructions and Characterization-

具有生物功能的自组装分子层-结构和表征-

• 批准号: 07044046
• 负责人: UOSAKI Kohei
• 金额: $ 4.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044046/
• 关键词: Self-assembled monolayr; Microelectrode; Scanning probe microscope; bioelectrochemistry; sensor; interfacial electron transfer

This research was conducted to construct and characterize the self-assembled monolayrs with biologically related functionalities. The following results were obtained.1. Microelectrodes suitabel for biosensor was designed and fabricated. The microelectrodes were characterized by atomic force microscope (AFM).2. Electrochemical characteristics of cytochorome-c at gold electrodes modified with mixed monolayrs of promoter and barrier molecules were investigated. The compositions of the monolayrs were determined by electrochemical quartz crystal microbalance (EQCM) and electrochemical measurements.3. Self-assembled process of promoter and barrier molecules was monitored by in situ scanning tunneling microscope (STM). In the case of barrier molecules, i.e., n-alkanethiols, the self-assembled monolayrs were formed through three stages, i.e., 1. liquid phase, 2. organized phase with stripe and mesh structures and 3. solid phase with close packed ROO<3>*ROO<3> structure.4. Usefulness of novel techniques such as sum frequency generation (SFG) and surface x-ray diffraction (SXRD) was evaluated.

本研究旨在构建具有生物相关功能的自组装单分子膜并对其进行表征。得到以下结果.设计并制作了适合于生物传感器的微电极。利用原子力显微镜（AFM）对微电极进行了表征.研究了细胞色素c在促进剂和屏障分子混合膜修饰的金电极上的电化学特性。用电化学石英晶体微天平（EQCM）和电化学方法测定了单分子膜的组成.利用扫描隧道显微镜（STM）原位监测了促进剂和阻挡层分子的自组装过程。在屏障分子的情况下，即，正烷硫醇，通过三个阶段形成自组装单层，即，1.液相，2.具有条纹和网状结构的组织相; 3.固相具有紧密堆积的ROO <3>*ROO<3>结构。评价了和频产生（SFG）和表面X射线衍射（SXRD）等新技术的可行性。

项目成果

I.Yagi: "In situ Optical Second Harmonic Generation Studies of Electrochemical Deposition of Tellurium on Polycrystalline Gold Electrodes" J.Electroanal.Chem.401. 95-101 (1996)

I.Yagi：“多晶金电极上碲电化学沉积的原位光学二次谐波产生研究”J.Electroanal.Chem.401。

T. Kondo: "pH Dependent Photoinduced Electron Transfer at the Gold Electrode Modified with Self Assembled Monolayers of Porphyrin-Mercaptoquinone Coupling Molecule" J. Electroanal. Chem.(in press). (1997)

T. Kondo：“用卟啉-巯基醌偶联分子自组装单层修饰的金电极上的 pH 依赖性光诱导电子转移”J. Electroanal。

T. Kondo: "Adsorption Behavior of Functionalized Ferrocenylalkane Thiols and Disulfide onto Au and ITO and Electrochemical Properties of Modified Electrodes: Effects of Acyl and Alkyl Groups Attached to the Ferrocene Ring" J. Electroanal. Chem.381. 203-20

T. Kondo：“功能化二茂铁烷硫醇和二硫化物在 Au 和 ITO 上的吸附行为以及修饰电极的电化学性能：连接到二茂铁环上的酰基和烷基的影响”J. Electroanal。

T.Kondo: "Photoelectrochemical Characteristics of Self-Assembled Monolayr of Porphyrin mercaptoquinone Coupling Molecules" T.hin Solid Films. 284. 652-655 (1996)

T.Kondo：“卟啉巯基醌偶联分子自组装单层的光电化学特性”T.hin 固体薄膜。

Y.Sato: "Electrochemical Properties of the 2-Mercaptohydroquinone Monolayr on a Gold Electrode. Effect of Solution pH,Adsorption Time and Concentration of the Modifying Solution" J.Electroanal.Chem.409. 145-154 (1996)

Y.Sato：“金电极上 2-巯基氢醌单层的电化学性质。溶液 pH 值、吸附时间和改性溶液浓度的影响”J.Electroanal.Chem.409。