Ditribution of bone matrix proteins and PTH recepter mRNA during ectopic bone formation induced by BMP

BMP诱导异位骨形成过程中骨基质蛋白和PTH受体mRNA的分布

• 批准号: 07457428
• 负责人: NAGATSUKA Hitoshi
• 金额: $ 4.22万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457428/
• 关键词: BMP; mRNA; in situ hybridization; bone matrix protein; collagen; non RI; PTH prcepter

Purtialy purified bovine BMP-collagen I : 1 W after implantation. Osteopontin mRNA expression was found mainly at the central area of the tissue. At higher magnification, strong osteopontin mRNA expression was seen in large ovary shaped cells Osteonectin mRNA expression was found in the central to the middle area of the tissue. Osteonectin mRNA expression seen in fibroblast-like cells. Osteocalcin mRNA expression could not be detected at 1W after implantatin. Type I collagen mRNA expresison was found in all area. 2 W after implantation. Strong osteopontin and osteonectin mRNA expression was found in almost all areas of the tissue. At higher magnificaiton, strong osteonectin mRNA expression was seen in chondrocytes, osteoblasts, and young osteocytes. Osteocalcin mRNA expression was found in osteoblasts and osteoxytes of the newly formed bone. Type II and X collagen mRNA expression was found in chondrocytes and few osteoblast. 3 W after implantation. Osteopontin mRNA expression was found … More in osteoblasts surrounding the trabecular-shaped woven bone. Strong osteonectin mRNA expression was found in osteoblasts an osteocytes. Osteocalcin mRNA expression was found in osteoblasts and young osteocytes. Type I collagen mRNA espression was found in osteoblasts and young osteocytes.rhBMP-2-IMB : In the heterotopic cartilage like tissue, Osteopontin, osteocalcin mRNA and Type II Collagen were detected in chondrocyte-like cells. Whileosteocalcin mRNA was absent in chondrocytes in the enodchondral ossification of the fetal rat tibia. These results suggest that the chondrocyte-like cells in the BMP induced cartilage like tissue demonstrate the two phase funciton of both chondrocytes and osteoblast. The inductive cartilage-like tissue may be deisgnated as chondro-osseous cells in stead of chondro-cytes. The heterotopic chondro-osseous tissue formation induced by rhBMP-2 is process that differs from that of normal endchondral ossification in respect to cell differentiation and function. Less

植入后1周，纯化牛BMP-胶原I。骨桥蛋白mRNA的表达主要集中在组织的中心区域。高倍镜下，在大的卵巢状细胞中观察到强烈的骨桥蛋白mRNA表达，在组织的中心至中间区域观察到骨连接素mRNA表达。在成纤维细胞样细胞中观察到骨连接素mRNA表达。骨钙素mRNA表达在照射后1 W时消失。I型胶原mRNA在各区域均有表达。植入后2 W。强骨桥蛋白和骨连接蛋白mRNA的表达被发现在几乎所有领域的组织。在更高的放大倍数下，在软骨细胞、成骨细胞和年轻骨细胞中观察到强的骨连接蛋白mRNA表达。骨钙素mRNA在新生骨的成骨细胞和骨氧化细胞中表达。软骨细胞和少数成骨细胞表达II型和X型胶原mRNA。植入后3 W。骨桥蛋白mRNA表达 ...更多信息 在小梁状编织骨周围的成骨细胞中。成骨细胞和骨细胞中骨粘连蛋白mRNA表达较强。骨钙素mRNA在成骨细胞和年轻骨细胞中表达。rhBMP-2-IMB：在异位软骨样组织中，骨桥蛋白、骨钙素mRNA和II型胶原在软骨样细胞中表达。而在软骨内成骨过程中，软骨细胞中骨钙素mRNA表达缺失。这些结果提示BMP诱导的类软骨组织中的类软骨细胞具有软骨细胞和成骨细胞的两相功能。诱导后的软骨样组织可分化为软骨-骨细胞而不是软骨细胞。rhBMP-2诱导的异位软骨-骨组织形成过程在细胞分化和功能方面不同于正常软骨内骨化过程。少

项目成果

Nagatsuka,H.: "Immunohistochemical study of cytokeratin patterns in follicular and plexiform ameloblastoma." J.Hard Tissue Biol.4. 50-56 (1995)

Nagatsuka,H.：“滤泡和丛状成釉细胞瘤细胞角蛋白模式的免疫组织化学研究。”

T.Takagi: "Effects of Bovine Amelogrnin and Enamelin on in vivo Mineralization in Gel" J.Hard Tissue Biol.Vol.5 (1). 15-22 (1996)

T.Takagi：“牛 Amelogrnin 和 Enamelin 对凝胶体内矿化的影响”J.Hard Tissue Biol.Vol.5 (1)。

石割裕三: "幼弱マウス下顎頭軟骨におけるI型,II型,X型コラーゲン遺伝子の発現-In situ hybridization方による検討-" 岡山歯学会雑誌. 15. 53-62 (1996)

Yuzo Ishiwari：“幼鼠下颌骨髁突软骨中 I 型、II 型和 X 型胶原基因的表达 - 原位杂交方法的研究 -”冈山牙科学会杂志 15. 53-62 (1996)。

N.Nagai: "Ultrasrtuctural study of inductive bone interface between bone morphogenic protein binding hydroxyapatite and heterotopic tissue" J.Hard Tissue Biol.Vol.5 (2). 102-109 (1996)

N.Nagai：“骨形态发生蛋白结合羟基磷灰石和异位组织之间诱导骨界面的超结构研究”J.Hard Tissue Biol.Vol.5 (2)。

Hong-In Shin: "The Effect of Enzymatic Treatment on Immunohistochemical Stainning of Collagens in Mandibular Condyle of Rats" J.Hard Tissue Biol.Vol.4(1). 8-14 (1995)

Hong-In Shin：“酶处理对大鼠下颌骨髁突胶原蛋白免疫组织化学染色的影响”J.Hard Tissue Biol.Vol.4(1)。