Cartilage-disruption factor involved in Kashin-Beck disease

大骨节病涉及的软骨破坏因子

• 批准号: 08044293
• 负责人: KATO Yukio
• 金额: $ 1.73万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044293/
• 关键词: chondrocyte; metalloproteinase; serum; gelatinase; animal model

1 The development of a Kashin-Beck Disease model (monkey) Monkeys were fed with water and food from the Kashin-Beck Disease area, and sera were obtained from these animals.2 sera were obtained from patients with Kashin-Beck Disease.3 Chinese and Japanese investigators discussed studies on Kashin-Beck Disease.4 MMP-9-inducing factors in sera from patients with Kashin-Beck Disease.Rabbit growth plate chondrocytes were incubated for 4 or 8 days in the presence of 5% control sera or 5% sera from patients with Kashin-Beck Disease. Gelatinese activity was estimated by gelatin zymography. No gelatinase activity was found in the cell layr, but MMP-2 were found in the conditioned medium. The sera from patients with Kashin-Beck Disease induced MMP-9 of 92kDa on day 4, and increased MMP-9 levels 3-to 4-fold on day 8 relative to control sera. These findings suggest that sera from patients with Kashin-Beck Disease contain factors that stimulate MMP-9 synthesis by growth plate chondrocytes.

1大骨节病模型（猴）的建立用来自大骨节病区的水和食物喂养猴，3中国和日本研究者讨论了大骨节病的研究。4大骨节病患者血清中MMP-9诱导因子，将兔生长板软骨细胞在5%对照血清或5%大骨节病患者血清存在下孵育4或8天。明胶酶谱法测定明胶酶活性。细胞层中无明胶酶活性，但条件培养液中有MMP-2表达。与对照血清相比，大骨节病患者血清在第4天诱导了92 kDa的MMP-9，并且在第8天使MMP-9水平增加3- 4倍。这些发现表明，大骨节病患者的血清中含有刺激生长板软骨细胞合成MMP-9的因子。

项目成果

Hashimoto K.,Noshiro M.,Ohno S.,Kawamoto T.,Satakeda H.,Akagawa Y.,Nakashima K.,Okimura A.,Ishida H.,Okamoto T.,Pan H.,Shen M.,Yan W.,Kato Y.: "Characterization of a cartilage-derived 66kDa protein (RGD-CAP/big-h3) that Binds to collagen" Biochem.Biophys.

桥本 K.、能代 M.、大野 S.、川本 T.、佐武田 H.、赤川 Y.、中岛 K.、冲村 A.、石田 H.、冈本 T.、潘 H.、沉明、严伟

Jikko A.,Hiranuma H.,Iawamoto M.,Kato Y.,Okada Y.,Fuchihata H.: "Effects of X irradiation on metabolism of proteoglycans" Radiation Res.146. 93-99 (1996)

Jikko A.、Hiranuma H.、Iawamoto M.、Kato Y.、Okada Y.、Fuchihata H.：“X 辐射对蛋白聚糖代谢的影响”辐射 Res.146。

Nakamura S.,Kamihagi K.,Satakeda H.,Katayama M.,Pan H.,Okamoto H.,Noshiro M.,Takahashi K.,Yoshihara Y.,Shimmei M.,Okada Y and Kato Y.: "Enhancement of SPARC/osteonectin synthesis in arthritic cartilage : its increased levels in Synovial Fluids from Patien

Nakamura S.、Kamihagi K.、Satakeda H.、Katayama M.、Pan H.、Okamoto H.、Noshiro M.、Takahashi K.、Yoshihara Y.、Shimmei M.、Okada Y 和 Kato Y.：“增强

Okimura A., Makihira S., and Kato Y.: "Cartilage-matrix protein synthesis is enhanced in arthritic cartilage." Arthritis Rheum. (in press).

Okimura A.、Makihira S. 和 Kato Y.：“关节炎软骨中软骨基质蛋白合成得到增强。”

Hashimoto K., Noshiro M., Ohno S., Kawamoto T., Satakeda H., Akagawa Y., Nakashima K., Okimura A., Ishida H., Okamoto T., Pan H., shen M., Yan W., Kato Y.: "Characterization of a cartilage-derived 66kDa protein (RGD-CAP/big-h3) that betainds to collagen"

桥本 K.、Noshiro M.、Ohno S.、川本 T.、佐竹田 H.、赤川 Y.、中岛 K.、冲村 A.、石田 H.、冈本 T.、潘 H.、沉 M.、严 W