Cartilage-disruption factor involved in Kashin-Beck disease

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1 The development of a Kashin-Beck Disease model (monkey) Monkeys were fed with water and food from the Kashin-Beck Disease area, and sera were obtained from these animals.2 sera were obtained from patients with Kashin-Beck Disease.3 Chinese and Japanese investigators discussed studies on Kashin-Beck Disease.4 MMP-9-inducing factors in sera from patients with Kashin-Beck Disease.Rabbit growth plate chondrocytes were incubated for 4 or 8 days in the presence of 5% control sera or 5% sera from patients with Kashin-Beck Disease. Gelatinese activity was estimated by gelatin zymography. No gelatinase activity was found in the cell layr, but MMP-2 were found in the conditioned medium. The sera from patients with Kashin-Beck Disease induced MMP-9 of 92kDa on day 4, and increased MMP-9 levels 3-to 4-fold on day 8 relative to control sera. These findings suggest that sera from patients with Kashin-Beck Disease contain factors that stimulate MMP-9 synthesis by growth plate chondrocytes.