The development of culture surfaces expressing functional groups that enhance isolation, proliferation and differentiation of mesenchymal stem cells.

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To improve adhesion in serum-free media, we developed self-assembled monolayer expressing OH and COOH at 60:40 (SAM-1). SAM-1 enhanced reorganization of adhesion plaques, adhesion and proliferation of human mesenchymal stem cells (MSC) in a serum-free medium. Furthermore, the expression of bone-related genes, along with calcium accumulation, was markedly enhanced in MSC cultures on SAM-1 after exposure to a serum-free osteogenesis-induction medium. In addition, SAM-1 enhanced anaerobic glycolysis and suppressed TCA cycle activity in MSC by down-regulating mitochondria-related-genes, which may be related to MSC stemness. These findings indicate that a combination of a serum-free medium and SAM is useful in basic studies on cell-substrate interactions and clinical studies towards MSC-based cell therapy.