ヒト甲状腺腫瘍細胞におけるMAPキナーゼカスケードに関する基礎研究とその臨床応用

人甲状腺肿瘤细胞MAP激酶级联的基础研究及其临床应用

• 批准号: 08671186
• 负责人: 對馬 敏夫
• 金额: $ 1.28万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671186/
• 关键词: 甲状腺癌; MAPキナーゼ; Raf-1; MAPキナーゼキナーゼ

ヒト甲状腺腫瘍では幾つかの癌遺伝子の異常、あるいは成長因子受容体の過剰発現などが報告されている。しかし細胞増殖に関与する情報伝達に関する異常は明らかにされていない。MAPキナーゼカスケードは各種細胞において細胞増殖や分化に重要な役割を果たす。そこでヒト甲状腺腫瘍組織におけるMAPキナーゼ(MAPK),その上流に存在するMAPKキナーゼ(MEK),Raf-1の発現、その活性を正常組織で比較した。甲状腺乳頭癌とろ胞癌組織では大部分でMAPK,MEKの発現量、活性ともに正常部に比して数倍に増加しており、これは免疫組織学的にも証明された。Raf-1活性には差がなかった。一方、良性の腺腫やバセドウ病組織ではMAPK,MEK,Raf-1ともに正常と差がなかった。培養した正常甲状腺細胞にIGF-Iなど種々の成長因子を添加した場合にも増殖に先んじてよMAPKが活性化された。癌組織ではGF-I,IGFBP-2 mRNAの発現増加も観察された。以上よりMAPKカスケードは腫瘍発育に関与する可能性が示唆される。今後の方針としてはMAPK,MEKの遺伝子自体に異常があるか否かを明らかにしたい。

The growth factor of thyroid cancer is very common, the growth factor of thyroid cancer is high, the growth factor of thyroid cancer is not normal. They are in love with each other. They are in love with each other. They are often told that they are not in love with each other. MAP has been used to cut the fruits of all kinds of cells for the purpose of cell differentiation and important service. The tissue of thyroid gland was detected by MAP enzyme assay (MAPK), the upstream of thyroid gland was detected by MAPK assay (MEK), and the activity of Raf-1 was detected by normal tissue assay. The amount of MAPK,MEK in the tissue of thyroid breast cancer was much higher than that in the normal tissue of thyroid breast cancer, and the quantity and activity of the normal tissue of thyroid breast cancer were higher than those of thyroid breast cancer. The activity of Raf-1 is different from that of others. On the other hand, the benign glandular disease tissue showed that the MAPK,MEK,Raf-1 was normal and the difference was negative. The growth factors of IGF-I in normal thyroid cells were cultured, and the growth factors of MAPK in normal thyroid cells were cultured in vitro. The cancer tissue can be detected by GF-I,IGFBP-2 mRNA. The above question "MAPK" and "possibility" indicate that you are instigated by the possibility. In the future, you will need to know if you are aware that you will not be able to MAPK,MEK your child.

项目成果

Osamu Isozaki: "Oncostatin M,a new potent inhibitor of iodine metabolism inhibits thyroid peroxidase gene expression but not DHA synthesis" Thyroid. 6 (印刷中). (1997)

Osamu Isozaki：“制瘤素 M，一种新型有效的碘代谢抑制剂，可抑制甲状腺过氧化物酶基因表达，但不会抑制 DHA 合成”，《甲状腺》6（出版中）。

Liu-Y.Z: "Insulin-like growth factor (IGFs) and IGF-binding proteins (IGFBPs) in doabetic pregnancy ; Relation ship to macrosomia" Endocrine Journal. 43. 221-231 (1996)

Liu-Y.Z：“双胎妊娠中的胰岛素样生长因子（IGF）和 IGF 结合蛋白（IGFBP）；与巨大儿的关系”内分泌杂志。

Megumi Miyakawa: "Carboxyterminal Propeptide type 1 procollagen (PICP) and C-terminal telopeptide of type 1 collagen as sensitive markers of bone metabolism" Endocrine Journal. 43. 701-708 (1996)

Megumi Miyakawa：“羧基末端前肽 1 型前胶原 (PICP) 和 1 型胶原 C 末端端肽作为骨代谢的敏感标记”内分泌杂志。

Isozaki O.: "Expression of insulin-like growth factor binding proteins (IGFBPs) in human thyroid papillary cancertissues" Endocrine Journal. 43 (sup). 597-598 (1996)

Isozaki O.：“人甲状腺乳头状癌组织中胰岛素样生长因子结合蛋白（IGFBP）的表达”内分泌杂志。