Regulation of NOS activity by the endogenous inhibitors and modification of hyperplastic vascular diseases with the inhibitors.
通过内源性抑制剂调节 NOS 活性并通过抑制剂改善血管增生性疾病。
基本信息
- 批准号:08672604
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
During the term of project, we obtained the following results : 1.Nicotine at plasma concentrations corresponding to the levels of human smokers would accelerate the intimal hyperplasia after endothelial denudation through the enhanced impairment of the EDRF/NO production, which might be brought about by the enhanced increases in LNMMA (N^G-monomethyl-L-arginine) and ADMA (N^G, N^G-dimethyl-L-arginine) concentrations as inhibitors for NO synthesis, and the enhanced increase in endothelin-1 as a potent mitogen in the vessel wall. 2.The NO synthesized endogenously from L-arginine might play a role for mediating relaxation of the bovine ciliary muscle and that the endogenous ADMA might be involved in inhibiting the biosynthesis of NO when there are increased intracellular concentrations of the methylarginine under certain circumstances. NO causes relaxation of the bovine ciliary muscle through the activation of guanylate cyclase and an increase in cyclic GMP level. 3.ADMA as an endogenous … More inhibitor of NO synthesis may play an important role for the pathogenesis in the hypertension associated with the experimental focal and segmental glomerulosclerosis induced by puromycin aminonucleotide in the rat. 4.Immunohistochemistry for proliferating cell nuclear antigen and Ki-67, both markers for proliferating cell nuclei, showed that tropoelastin transcripts and elastin formation increased when smooth muscle cells enter quiescence after the end of the proliferative phase in the intima, suggesting that elastin synthesis and smooth muscle cell proliferation are tughtly regulated during the repair of arterial wall injury. 5.The expression of osteopontin mRNA in rabbit neointima after balloon endothelial denudation was associated with proliferating smooth muscle cells. In primary cultures of arterial smooth muscle cells.osteopontin mRNA was expressed in cells that had transferred to the synthetic state (G_<1B>, S and G_2 + M), and not in cells in either the contractile (G_0 phase) or intermediate states (G_<1A> phase), indicating that osteopontin mRNA expression provides a useful marker that can be applied to distiguish the phenotypic properties of vascular smooth muscle cells. 6.Increases in p53 protein and p53 gene transcript levels would be closely linked to the proliferation of smooth muscle cells in the thickened intima, and play a key role in the regulation of cell proliferation during the repair process after arterial wall injury. Less
在项目实施期间,我们取得了以下成果:1.吸烟者血浆尼古丁浓度与内皮剥脱后的内膜增生密切相关,尼古丁可通过增强内皮细胞LNMMA的表达,抑制内皮细胞EDRF/NO的产生,从而促进内膜增生。(N^G-单甲基-L-精氨酸)和ADMA(N^G,N^G-二甲基-L-精氨酸)浓度作为NO合成的抑制剂,以及内皮素-1作为血管壁中有效的有丝分裂原的增强增加。2.内源性L-精氨酸合成的NO可能在介导牛睫状肌松弛中起作用,而当细胞内甲基精氨酸浓度增加时,内源性ADMA可能参与抑制NO的生物合成。NO通过激活鸟苷酸环化酶和增加环GMP水平引起牛睫状肌松弛。3. ADMA作为内源性 ...更多信息 NO合成抑制剂可能在嘌呤霉素所致大鼠局灶节段性肾小球硬化相关高血压的发病机制中起重要作用。4.增殖细胞核抗原和Ki-67的免疫组织化学,这两个标志物的增殖细胞核,表明,原弹性蛋白转录和弹性蛋白形成增加时,平滑肌细胞进入静止期后,在内膜增殖阶段结束,这表明弹性蛋白的合成和平滑肌细胞增殖的动脉壁损伤的修复过程中受到严格的监管。5.球囊内皮剥脱术后兔血管内膜骨桥蛋白mRNA的表达与平滑肌细胞增殖有关。在原代培养的动脉平滑肌细胞中,骨桥蛋白mRNA在进入合成状态(G_<1B>、S和G_2 + M)的细胞中有表达,而在收缩状态(G_0期)和中间状态(G_期)的细胞中无表达<1A>,表明骨桥蛋白mRNA的表达可作为区分血管平滑肌细胞表型的一个有用的标志。6. p53蛋白和p53基因转录水平的增加与增厚内膜中平滑肌细胞的增殖密切相关,在动脉壁损伤后修复过程中对细胞增殖起关键调节作用。少
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Aoyagi M,Yamamoto M,Azuma H et al.: "Smooth muscle cell proliferation,elastin formation,and tropoelestin transcripts during the development of intimal thickening in rabbit carotid arteries after endothelial denudation" Histochemistry Cell Biology. (in pre
Aoyagi M、Yamamoto M、Azuma H 等人:“内皮剥脱后兔颈动脉内膜增厚发育过程中的平滑肌细胞增殖、弹性蛋白形成和原弹性蛋白转录”组织化学细胞生物学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hamasaki H,Sato J.et al.: "Effect of nicotine on the intimal hyperplasia after endothelial removal of the rabbit carotid artery" General Pharmacology. 28(4)(in press). (1997)
Hamasaki H,Sato J.等人:“尼古丁对兔颈动脉内皮去除后内膜增生的影响”一般药理学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Aoyagi M,Yamamoto M,Azuma et al.: "Expression of p53 protein and p53 gene transcripts in rabbit carotid arteries after balloon denudation" Histochemistry Cell Biology. (in press). (1997)
Aoyagi M、Yamamoto M、Azuma 等人:“球囊剥脱后兔颈动脉中 p53 蛋白和 p53 基因转录物的表达”组织化学细胞生物学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Aoyagi M, Yamamoto M, Azuma H.et al.: "Smooth muscle cell proliferation,elastin formation,and tropoelestin transcripts furing the development of intimal thickening in rabbit carotid arteries after endothelial denudation." Histochemistry and Cell Biology.
Aoyagi M、Yamamoto M、Azuma H.等人:“平滑肌细胞增殖、弹性蛋白形成和原弹性蛋白转录物促进内皮剥脱后兔颈动脉内膜增厚的发展。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sato J,Masuda H,Tamaoki S,Hamasaki H,Ishizaka K,Matsubara O,Azuma H.: "Possible involvement of endogenous asymmetrical dimethyl-arginine in the hypertension associated with puromycin nephrosis in the rat." British Journal of Pharmacology. (in press). (199
Sato J、Masuda H、Tamaoki S、Hamasaki H、Ishizaka K、Matsubara O、Azuma H.:“内源性不对称二甲基精氨酸可能参与与大鼠嘌呤霉素肾病相关的高血压。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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AZUMA Hiroshi其他文献
AZUMA Hiroshi的其他文献
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{{ truncateString('AZUMA Hiroshi', 18)}}的其他基金
Detection of molecules involved in the induction of immunosuppressive function of MDSC-like cells induced by liposome
脂质体诱导MDSC样细胞免疫抑制功能相关分子的检测
- 批准号:
25461578 - 财政年份:2013
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis for novelty in floral scent chemistry of natural hybrid between closely related species.
密切相关物种之间自然杂交花香化学的新颖性分析。
- 批准号:
23570116 - 财政年份:2011
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Acceleration of intinul hyperplasia by the enhanced arginase activity due to hyperglycemia
高血糖导致精氨酸酶活性增强,加速血管内增生
- 批准号:
14572152 - 财政年份:2002
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cultural Scripts as Frameworks for Understanding, Predicting and Evaluation of Actions of Others.
文化脚本作为理解、预测和评估他人行为的框架。
- 批准号:
14310062 - 财政年份:2002
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Possible involvement of facilitated aldose reductase activity in the accelerated vascular remodeling in rabbits with alloxan-induced hyperglycemia
促进醛糖还原酶活性可能参与四氧嘧啶诱导的高血糖兔加速血管重塑
- 批准号:
12672209 - 财政年份:2000
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
influencing inter-cultural and ntra-cultural variability
影响跨文化和文化内的变异性
- 批准号:
11410036 - 财政年份:1999
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A Cognitive Construct of Motivation in JAPAN
日本动机的认知结构
- 批准号:
07451035 - 财政年份:1995
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of interleukin 6 and its soluble receptor in cerebrospinal fluid
脑脊液中白细胞介素6及其可溶性受体的分析
- 批准号:
06670758 - 财政年份:1995
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
A cross-cultural study of moral judgment made under insufficient information
信息不足下道德判断的跨文化研究
- 批准号:
04451029 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
A computerized system for training workers in the diagnosis and remedial teaching of children with cognitive development problems
用于培训工作人员对患有认知发展问题的儿童进行诊断和治疗教学的计算机化系统
- 批准号:
04558040 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
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ADMA通过NOX2/ROS/TGF-β通路调控视网膜纤维血管增殖的作用和机制
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SANDIA: Supplementing L-citrulline to overweight late Asthma oNset phenotypes to increase airway L-arginine/ADMA ratio and Improve Asthma control
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ADMA as a novel therapeitic target for CKD patitents with PAD
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17K16096 - 财政年份:2017
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