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CDNA representation analysis of primary and metastatic colon cans

原发性和转移性结肠罐的 cDNA 代表性分析

基本信息

批准号：
10670513
负责人：
NAKAMURA Tetsuo
金额：
$ 2.11万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670513/
关键词：
RDA cDNA Subtraction Colon Reg-1-α GW112 Annexin-1 RDA法大腸癌

项目摘要

In this study, cDNA representation difference analysis (RDA) was used to identify candidate genes selectively expressed in liver metastasis of colon cancer which are not expressed in primary colon cancer. In RDA with the primary cancer as "tester" and an excess amount of the metastasis as "drivers", no discrete bands were seen. In RDA with the metastasis as "tester" and the primary cancer as "drivers", 8 candidate genes were found. Of 8 candidate genes, four were found to be identical to Reg 1α, GW112, Annexin-1 and Bax by DNA sequencing. Two were found to be the genes submitted to GenBank (one was the gene cloned in cosmid L219 and the other was the gene on chromosome 16p13), although the function of these genes were unkown. The other two clones were not identical to any of the genes submitted to GenBank. Of these, we further examined for Reg 1α, Annexin-1 and Bax functions of which are reported, and for GW112, which is the dominant species in RDA.During further examination of these genes, we found that the expression of these genes were not restricted to metastasis, but closely related to the inflammation of colon. We showed the relation between Bax gene expression and inflammation of the colon and the relation between leg 1α, Annexin-1 and GW112 gene expression and inflammation of the colon in detail in the report.

本研究采用cDNA表达差异分析（RDA）来鉴定在结肠癌肝转移中选择性表达而在原发性结肠癌中不表达的候选基因。在 RDA 中，原发癌作为“测试者”，过量的转移癌作为“驱动者”，没有看到离散的条带。在以转移癌为“测试者”、原发癌为“驱动者”的RDA中，发现了8个候选基因。通过 DNA 测序，发现 8 个候选基因中，有 4 个与 Reg 1α、GW112、Annexin-1 和 Bax 相同。发现两个是提交给GenBank的基因（一个是克隆在粘粒L219中的基因，另一个是染色体16p13上的基因），尽管这些基因的功能尚不清楚。另外两个克隆与提交给 GenBank 的任何基因都不相同。其中，我们进一步检查了已报道的Reg 1α、Annexin-1和Bax功能，以及RDA中的优势种GW112。在对这些基因的进一步检查过程中，我们发现这些基因的表达不仅限于转移，还与结肠炎症密切相关。我们在报告中详细展示了Bax基因表达与结肠炎症的关系，以及leg 1α、Annexin-1和GW112基因表达与结肠炎症的关系。