Correlation between Apoptosis, oncogene, SOD and radiation response
细胞凋亡、癌基因、SOD 与放射反应的相关性
基本信息
- 批准号:10670881
- 负责人:
- 金额:$ 2.05万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1 Correlation between Bax, bcl-2 protein expression and radiation-induced apoptosisBax bcl-2 protein expression correlate with radiation-induced apoptosis on 21 cases with the cervical cancer, including 11 cases of stage 2, 8 cases of stage 3, and 2 cases of stage 4 was investigated. Apoptosis was detected by using Apoptag (Oncor), and Apoptotic Index (AI) was calculated. Bax and bcl-2 protein expressions were detected with both anti-Bax antibody (Oncor) and anti-bcl-2 antibody (DAKO)immunohistochemically.RESULT : Apoptosis was 0.02% before irradiation, and it significantly increased up to 1.2% at 9Gy. The Dositive rate of Bax was 15% before irradiation, and it increased up to 60% at 9Gy. While that of bcl-2 was 15% before irradiation, and it increased to 25% at 9Gy which was no significant increase. Additionally, a significant correlation was observed between Bax expression and apoptosis of the tumor at 9Gy.CONCLUSION : Bax protein expression significantly correlates with radiation-induced apoptosis of cervical cancer.2 Correlation between p53, p21 protein expression and radiation-induced apoptosisCorrelation between p53, p21 tumor expression and radiation-induced apoptosis were investigated. Before irradiation, the mean AI of p53 and p21 were 6.60% and 9.24% respectively. Then after 9Gy the mean AI of p53 and p21 significantly increased to 13.9% and 18.0% respectively. A significant positive correlation appeared between p53 protein index and p21 protein index before irradiation. Mean AI, p53 index, and p21 index were 0.98%, 7.33% and 8.77% respectively. As for radiation-induced AI, a significant negative correlation was observed between both AI and p53 or p21. Moreover, a significant positive correlation was observed between both p53 and p21.CONCLOSION : The cascade of p53 and p21 act as apoptosis inhibitory action in cervical cancer.
1 Bax、bcl-2蛋白表达与辐射诱导凋亡的相关性研究21例宫颈癌患者Bax、bcl-2蛋白表达与辐射诱导凋亡的相关性,其中2期11例,3期8例,4期2例。采用凋亡标记(Apoptag, Oncor)检测细胞凋亡,计算细胞凋亡指数(Apoptosis Index, AI)。用抗Bax抗体(Oncor)和抗bcl-2抗体(DAKO)免疫组化检测Bax和bcl-2蛋白的表达。结果:辐照前细胞凋亡率为0.02%,辐照9Gy后细胞凋亡率显著升高至1.2%。辐照前Bax阳性率为15%,辐照9Gy时阳性率高达60%。而bcl-2在辐照前为15%,在9Gy时升高至25%,但无明显升高。此外,9Gy时Bax表达与肿瘤细胞凋亡有显著相关性。结论:Bax蛋白表达与辐射诱导宫颈癌细胞凋亡有显著相关性研究p53、p21蛋白表达与辐射诱导细胞凋亡的相关性。放疗前,p53和p21的平均AI分别为6.60%和9.24%。9Gy后,p53和p21的平均AI分别显著升高至13.9%和18.0%。辐照前p53蛋白指数与p21蛋白指数呈显著正相关。平均AI、p53指数、p21指数分别为0.98%、7.33%、8.77%。在辐射诱导的AI中,AI与p53或p21呈显著负相关。此外,p53和p21之间存在显著的正相关。结论:p53和p21的级联表达在宫颈癌中具有抑制细胞凋亡的作用。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Niibe Y,Nakano T,et al.: "Inverse correlation between radiation-induced apoptosis and p53,p21/WAF-1 in cervical cancer patients treated by radiotherapy." In press Int.J.Radiato.Oncol Biol Phys.1999.
Niibe Y、Nakano T 等人:“接受放射治疗的宫颈癌患者中放射诱导的细胞凋亡与 p53、p21/WAF-1 之间呈负相关。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ohno T,Nakano T, et al.: "Bax Protein Expression Correlates with Radiation-induced Apoptosis in Radiation Therapy for Cervical Cancer"Cancer. 83. 103-110 (1998)
Ohno T、Nakano T 等人:“Bax 蛋白表达与宫颈癌放射治疗中放射诱导的细胞凋亡相关”癌症。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Niibe Y.,Nakano T.et al.: "Inverse correlation between radiation-induced apoptosis and p53,p21/WAF 1 in cervical cancer patients treated by radiotherapy"Int.J.Radiato.Oncol Biol Phys.. 44. 297-303 (1999)
Niibe Y.、Nakano T.等人:“接受放射治疗的宫颈癌患者中放射诱导的细胞凋亡与 p53、p21/WAF 1 之间呈负相关”Int.J.Radiato.Oncol Biol Phys.. 44. 297-303
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Abe A,Nakano T, et al.: "Clinical evaluation of serum and immunohistochemical expression of SCC and CA19-9 in radiation therapy for cervical cancer"Anti Cancer Research. 19. 829-836 (1999)
Abe A、Nakano T 等人:“宫颈癌放射治疗中 SCC 和 CA19-9 的血清和免疫组织化学表达的临床评价”抗癌研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Nakano T, Oka K, et al: "Immunohistochemical prediction of radiation response and local control in radiation therapy for cervical cancer"Cancer Detect Prevent. 22. 120-128 (1998)
Nakano T、Oka K 等人:“宫颈癌放射治疗中放射反应的免疫组织化学预测和局部控制”癌症检测预防。
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- 影响因子:0
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慢性束缚应激后中脑灰质中央 GFAP 和谷氨酸转运蛋白减少
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OHMICHI Yusuke;OHMICHI Mika;SAKURAI Hiroki ;NAKANO Takashi;ASAMOTO Ken;USHIDA Takahiro;SATO Jun;井辺弘樹 - 通讯作者:
井辺弘樹
NAKANO Takashi的其他文献
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{{ truncateString('NAKANO Takashi', 18)}}的其他基金
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短寿命放射性同位素供应平台
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Management of environment to secure the quality of educators in the introduction of the new-curriculum
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Modification of radiation sensitivity in radiation therapy by combination of molecular target therapy
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16390334 - 财政年份:2004
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