Isolation of colorectal cancer-related genes, induced by β-catenin activation.

为了解决癌变的机制，我们已经确定了被活化的β-连环蛋白诱导的基因。将缺乏完整外显子3的突变型β-catenin cDNA导入来源于小鼠sc组织的L细胞。在这些细胞（设计的LM细胞）中检测到异常β-catenin的高表达，而在转染野生型β-catenin的细胞（设计的LN细胞）中，其表达水平与亲本细胞相似。LM细胞的形态形态更偏向于立方体，生长培养基中血清浓度较低。LM细胞的多层生长模式也被确认。此外，通过差异展示的方法，鉴定了6个被活化β-catenin诱导或抑制表达的基因。2到6个是已知基因，其余4个是新基因。

To resolve the mechanism for carcinogenesis, we have identified genes which were induced by activated β-catenin. A mutated-form of β-catenin cDNA that lacked an entire exon 3 was introduced into L cells derived from murine S.C.tissue. High expression level of aberrant β-catenin was detected in those cells (designed LM cells), whereas in cell transfected wild-type β-catenin (designed LN cells) showed similar level as those of parental cells. The morphological appearance of LM cell was changed to a more cuboidal shape and required a lower concentration of serum in the growth medium. Multilayer growth pattern of LM cells was also recognized. Furthermore, by the method of differential display, six genes of which expression was induced or suppressed by activated β-catenin were identified. Two to six were known genes and the remaining four were novel.