Isolation of colorectal cancer-related genes, induced by β-catenin activation.

为了解决致癌的机制，我们已经鉴定了由激活的β-连环蛋白诱导的基因。将缺失完整外显子3的突变型β-catenin基因导入小鼠胚胎干细胞来源的L细胞。在这些细胞(设计的LM细胞)中检测到异常的β-catenin的高表达，而在野生型β-catenin的细胞(设计的LN细胞)中检测到与亲本细胞相似的水平。细胞形态呈立方体状，且对血清浓度要求较低。多层细胞生长模式也被认为是LM细胞。此外，通过差异显示的方法，鉴定了6个被激活的β-连环蛋白诱导或抑制表达的基因。有两到六个是已知基因，其余四个是新基因。

To resolve the mechanism for carcinogenesis, we have identified genes which were induced by activated β-catenin. A mutated-form of β-catenin cDNA that lacked an entire exon 3 was introduced into L cells derived from murine S.C.tissue. High expression level of aberrant β-catenin was detected in those cells (designed LM cells), whereas in cell transfected wild-type β-catenin (designed LN cells) showed similar level as those of parental cells. The morphological appearance of LM cell was changed to a more cuboidal shape and required a lower concentration of serum in the growth medium. Multilayer growth pattern of LM cells was also recognized. Furthermore, by the method of differential display, six genes of which expression was induced or suppressed by activated β-catenin were identified. Two to six were known genes and the remaining four were novel.