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Developmental changes and eddect of soft diet on enzyme activites and on morphology of rat masseter and cortex mitochondria

软食对大鼠咬肌和皮质线粒体酶活性及形态的发育变化及影响

基本信息

批准号：
10671722
负责人：
SATO Iwao
金额：
$ 1.92万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671722/
关键词：
Mitochondria Masseter SDH NADH Dehydrogenase Enzyme activities Ultrastructure Cristae Soft diet NADH脱水素酵素酵素活性 NADH

项目摘要

1) Development of masseter muscleDevelopment of mitochondria was mostly gradual in 0- to 15-day postnatal rats. However, a notable increase was found in the CSA of mitochondria between days 10 and 15 in the neonatal rat. The change in feeding behavior from suckling to mastication was well-correlated with an increase of mitochondrial enzyme activites, also supported by the early development of mitochndria, especially with the initiation of growth in the corss-sectional area of the mitochndria.2) Development of rat cortexThe increases of oxidase activites are approximately correlated with the enhancement of complexity of mitochondrial cristae observed by TEM, which is remarkable after 10 days postnatal of rat cortex. The number of mitochondrion per cell nearly doubles and area of the mitochondrion reduced to one-half between 5 days and 10 days. The CSAs of mitochondrion and cell increase about twice from 10 days to 15 days, stabilize at 21 days, indicating the mitochondria and cell grow synchronously between 10 days and 21 days.3) Effect of soft dietThe decreased of number mitochondria concern with the reduction of these enzyme activities. However, a notable increase was found in succinate-OィイD22ィエD2 oxidoreductase activity from 26 days to 60 days. An constant in the number of mitochondria per muscle fiber did not seem to occur during these periods. The change in feeding stress to mastication was correlated with an decrease of mitochondrial enzyme activites, especially with the decreased in the number of the mitochondria per area with long-term soft diet.

1)咬肌的发育大鼠出生后0 ~ 15天线粒体的发育基本是渐进的。然而，在第10天至第15天，新生大鼠线粒体CSA明显增加。从哺乳到咀嚼的摄食行为的变化与线粒体酶活性的增加密切相关，这也与线粒体的早期发育，特别是线粒体横截面积的开始生长有关。氧化酶活性的增加与透射电镜观察到的线粒体嵴复杂性的增加大致相关，在大鼠出生后10天的皮层中，这种增加是显著的。在5天至10天期间，每个细胞的线粒体数量几乎增加了一倍，线粒体面积减少了一半。10 ~ 15天，线粒体和细胞的csa增加约2倍，在21天稳定，说明10 ~ 21天线粒体和细胞同步生长。3)软饲粮的影响：线粒体数量的减少与这些酶的活性降低有关。然而，从26天到60天，琥珀酸- o γ γ γ - 22 γ γ - D2氧化还原酶活性显著增加。在这些时期，每条肌纤维的线粒体数量似乎没有保持不变。饲喂对咀嚼的应激变化与线粒体酶活性的降低有关，特别是与长期软饲导致单位面积线粒体数量的减少有关。