Expression of tenascin-Xon rat TMJ in functional property

Tenascin-Xon大鼠TMJ功能特性的表达

• 批准号: 13671918
• 负责人: SATO Iwao
• 金额: $ 0.9万
• 依托单位: Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671918/
• 关键词: Extracellular matrix; Tenascin X; TMJ; Soft diet; mRNA; PCNA; Apoptosis; Masseter; ラット; テネイシンスーパーファミリー; テネイシンC

The tenascin family including tenascin-C, -X, -R, and N is known as one of extracellular matrix. Mainly tenascin-X, which is detected in ligaments and tendon of the joint hyperextensibility, and tenascin-C, which is associated with cell adhesion and conjugation, are observed. in this study, we investigated the transcription and translation levels of tenascin-C and -X in rat TMJ and masseter muscle during TMJ forming period, and on soft diet.1.Distribution of tenascin during development : Tenascin-C and X were detected in masseter muscle, connective tissue, and the bone matrix in the mandibular condyle and the mandibular fossa, on 18-days gestation. On 20-days gestation, tenascin-X was detected in the upper side of the TMJ disk in contrast to that of tenascin-C. During postnatal period, while tenascin-C was detected on masseter muscle and the middle region of the disk, tenascin X was detected mainly on the myotendinous junction, middle and outer regions of the disk and the bone matrix i … More n the mandibular fossa. These results suggest that in the period from 20-days gestation to postnatal, the localization of the tenascin family changes, at the same time cell movement and adhesion take place, resulting in the developed TMJ. Tenascin-C mRNA expressed more than tenascin-X mRNA in the newborn period, although the expression level of these had no difference in other periods. This suggests that in the newborn period these two play different roles. it seems according to the observation on the different localization of tenascin-C and -X in the TMJ disk that tenascin-C and -X are specially related to TMJ disk development.2.The effect of a soft diet : After weaning, one group was fed on a soft diet, while the other group was fed on a normal diet for three weeks. Tenascin-C was detected mainly on anterior and posterior regions of the disk in both groups. Especially the strong reaction was observed on the middle region of the disk in soft diet group in the first week. Tenascin-X was revealed the same region of tenascin-C in both groups and in soft diet group, the strong reaction was observed on the upper side of the disk in the first week. The PCNA-positive cell was observed on cartilage of mandibular condyle and inner and outer region of the disk in control group. In soft diet group, the number of the PCNA-positive cells decreased in the first week, then increased in the third week. The apoptosis-positive cell was observed on the bone matrix in the mandibular fossa and the mandibular condyle, in both groups in soft diet group the apoptosis-positive cells was also observed on cartilage of the condyle in the second week, and on cartilage cells and epithelial tissues in the eighth week. This suggests that the distribution of tenascin -C and -X, and cells and tissues composed of TMJ were changed by the stress of a soft diet for three weeks, and that mechanical stress causes cell movement and separation. Less

腱生蛋白家族包括腱生蛋白-C、腱生蛋白-X、腱生蛋白-R和腱生蛋白-N，是已知的细胞外基质之一。主要是腱生蛋白-X，其在关节过度伸展的韧带和肌腱中检测到，以及腱生蛋白-C，其与细胞粘附和结合相关。本研究以大鼠TMJ和咬肌为研究对象，研究了TMJ形成期和软食条件下TMJ和咬肌组织中Tenascin-C和Tenascin-X的转录和翻译水平。1.发育过程中Tenascin的分布：妊娠18 d时，Tenascin-C和Tenascin-X在大鼠咬肌、结缔组织、下颌骨髁突和下颌窝的骨基质中均有表达。在妊娠20天，在TMJ盘的上侧检测到生腱蛋白-X，而生腱蛋白-C。生后，Tenascin-C主要表达于咬肌和盘中部，Tenascin-X主要表达于肌腱连接处、盘中部和外侧以及骨基质中。 ...更多信息 在下颌窝。这些结果表明，在妊娠20天至出生后的这段时间内，tenascin家族的定位发生了变化，同时细胞发生了运动和粘附，导致了TMJ的发育。在新生儿期，Tenascin-C mRNA的表达高于Tenascin-X mRNA，而在其他时期两者的表达水平无差异。这表明，在新生儿时期，这两个发挥不同的作用。根据对Tenascin-C和Tenascin-X在颞下颌关节盘中不同定位的观察，推测Tenascin-C和Tenascin-X与颞下颌关节盘的发育有特殊的关系。2.软食的影响：断奶后，一组饲喂软食，另一组饲喂普通饲料，为期三周。在两组中，Tenascin-C主要在椎间盘的前部和后部区域被检测到。特别是软食组在第1周时，椎间盘中部出现较强的反应。Tenascin-X与Tenascin-C在两组中的表达相同，软食组在第一周时在盘的上侧观察到强烈的反应。对照组髁状突软骨及关节盘内外区可见PCNA阳性细胞。软食组PCNA阳性细胞数在第1周减少，第3周增加。在两组动物中，软食组第2周在髁突软骨上也有阳性细胞，第8周在软骨细胞和上皮组织上也有阳性细胞。这表明，腱生蛋白-C和-X的分布，以及TMJ的细胞和组织组成的软质饮食的压力，三个星期的变化，机械应力引起细胞的运动和分离。少

项目成果

I.Sato, S.Kunimoto, M.Sunohara: "Distribution of tenascin-X and expression of tenascin-X mRNA in post-natal rat TMJ"J.D.R.. 82. B-372 (2003)

I.Sato、S.Kunimoto、M.Sunohara：“产后大鼠 TMJ 中生腱蛋白-X 的分布和生腱蛋白-X mRNA 的表达”J.D.R.. 82. B-372 (2003)

Iwao SATO, Mikami A: "Distribution of tenascin-C and tenasoin-X in rat TMJ developemnt"Okajimas Folia Anat.Jpn.. 80(2-3). 57-62 (2003)

Iwao SATO，Mikami A：“大鼠 TMJ 发育中腱蛋白-C 和腱索因-X 的分布”Okajimas Folia Anat.Jpn.. 80(2-3)。

T.YATSU, K.KURIHARA, I.SATO: "Distrubution of tenascin, fibronectin, and collagen type I and microradiography in the mental symphyseal region of the human fetus."J.D.R.. 82. B-97 (2003)

T.YATSU、K.KURIHARA、I.SATO：“人类胎儿颏颏联合区域中生腱蛋白、纤连蛋白和 I 型胶原蛋白的分布以及显微放射照相术。”J.D.R.. 82. B-97 (2003)

Iwao SATO, Mikami A. et al.: "Distribution of tenascin-C and tenascin-X in rat TMJ development"Okajimas Folia Anat.Jpn.. 80(2-3). 57-62 (2003)

Iwao SATO，Mikami A.等：“大鼠TMJ发育中肌腱蛋白-C和肌腱蛋白-X的分布”Okajimas Folia Anat.Jpn..80(2-3)。

SATO I., S.KUNIMOTO, M.SUNOHARA: "Distribution of tenascin-X and expression of tenascin-X mRNA in post-natal rat TMJ"J.D.R.. 82. B-372 (2003)

SATO I.、S.KUNIMOTO、M.SUNOHARA：“产后大鼠 TMJ 中生腱蛋白-X 的分布和生腱蛋白-X mRNA 的表达”J.D.R.. 82. B-372 (2003)