Ultrastructure and Distribution of the Extracellular Matrix in the Collagen Fibers of the Masticatory Muscles in the Rat Postnatal Period.

摘要：采用电泳法观察大鼠产后（2周）咀嚼肌胶原纤维的分布。采用组织化学、电镜和免疫组织化学方法观察大鼠产后（2周）咀嚼肌胶原纤维的分布。1) SDS-PAGE分析表明，13 d时颞肌肌球蛋白重链上方的3条弱反应带中的第3条消失，但肌纤维组织化学分析显示琥珀酸脱氢酶活性特异性反应较强。2)免疫组织化学分析表明，细胞外基质成分在肌外膜（l型胶原）、肌周膜（l型胶原）和肌内膜（层粘连蛋白）中的分布与第12 ~ 13.3天的代谢能力有关。3)扫描电镜分析表明，肌肉纤维被薄的网状纤维包围。胶原纤维数量逐渐增加，形成网状，形成粗束。最后，粗大的胶原纤维束排列整齐。4)透射电镜分析表明，卫星细胞细胞器在各阶段均有分化。上述结果提示，细胞外基质、卫星细胞和生长因子对大鼠咀嚼肌发育有影响。在这些阶段，大鼠咀嚼肌肌球蛋白表现出特定的电泳模式。

Summary : The distribution of collagen fibers of rat masticatory muscles during the postnatal period (for 2 weeks), was investigated by electrophoresis. The distribution of collagen fibers of rat masticatory muscles during the postnatal period (two weeks), was investigated by histochemistry and electron microscopy, and immunohistochemistry.1) Analysis SDS-PAGE by in dicated that the third band of three weakly reactive bands above the heavy chain of myosin disappeared in the temporal muscle on 13 days, however, histochemical analysis of the muscle fibers revealed the reaction specific for the succinic dehydrogenase activity was strong. 2) lmmunohistochemical analysis indicated that the distribution of the components of the extracellular matrix in the epimysium (type l collagen), perimysium (type l collagen), and endomysium (laminin) was related to the metabolic capacity from days 12 to 13.3) Scanning electron microscopic analysis indicated that the muscle fibers were surrounded by thin reticular fibers. The number of collagenous fibers had gradually increased, and they formed networks formed thick bundles. Finally, thick bundles of thick collagen fibers were arranged regularly. 4) Transmission electron microscopic analysis indicated that the cell organelle in the satellite cell differentiated in each stage.These results suggested that the extracellular matrix, satellite cell with growth factors affect to the development of rat masticatory muscles on days 12 to 13. At these stages, the myosin of rat masticatory muscles displays specific electrophoretic patterns.