Reconstitution of Hair Follicles

毛囊重建

• 批准号: 11671786
• 负责人: TAKEDA Akira
• 金额: $ 1.79万
• 依托单位: KITASATO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671786/
• 关键词: Rats; Hair; Culture; 毛包

In recent years, several in vitro methods have been used for research of hair biology. The predominant methods could be classified into groups : culturing of individual follicular cells and maintaining of intact follicles. Moreover, a third method was described, in which dissociated cells derived from some tissues and organs could form the original tissues or organs again by rotation culture under proper conditions. We have previously reported reconstruction of hair follicles from a single cell suspension of rat fetal upper lip by a two-step culture method consisting of rotation and floatation cultures. Rotation provided sorting out of the cells and floatation facilitated histodifferentiation. In the present study, we added grafting procedures to the previous method to see whether cell aggregates obtained by this method were graftable, and whether the grafting promoted histodifferentiation. The aggregates before and after floatation step were subjected to grafting. Differentiation of hair follicles comparable to those in vivo was confirmed ten days after grafting. No difference in the degree of differentiation was observed between the two kinds of grafts. Thus the grafting procedure resulted in a significant enhancement of histodifferentiation even when aggregates obtained after the floatation step were grafted.An ultimate goal of our research is increasing the number of hair follicles in clinical human cases. For that aim, we must solve the following two problems. One problem is how the number of the cells which have a capacity of reforming the follicles can be increased. The second is how the increased folicles can be returned to patients. In this study, we focused on the second problem.

近年来，已有几种体外方法用于毛发生物学的研究。主要的方法可分为培养单个卵泡细胞和维持完整卵泡两大类。此外，本文还介绍了第三种方法，即从某些组织和器官中分离出来的细胞，在适当的条件下，通过旋转培养，可以重新形成原始组织或器官。我们以前报道过用两步培养方法从大鼠胎儿上唇的单细胞悬浮液中重建毛囊，包括旋转和漂浮培养。旋转有助于细胞的分选，漂浮有助于组织分化。在本研究中，我们在之前的方法基础上增加了移植程序，观察这种方法获得的细胞聚集体是否可移植，以及移植是否促进了组织分化。对浮选前后的骨料进行接枝处理。移植10天后，证实了与体内毛囊相当的毛囊分化。两种移植物的分化程度无差异。因此，即使移植了漂浮步骤后获得的聚集体，移植过程也能显著增强组织分化。我们研究的最终目标是增加临床人类病例中毛囊的数量。为此，我们必须解决以下两个问题。一个问题是如何增加有能力改造卵泡的细胞的数量。第二是如何将增加的卵泡返回给患者。在本研究中，我们重点研究了第二个问题。