Analysis of TH1/TH2 imbalance in Shogren's syndrome and its strategic control

本研究旨在分析TH1/TH2失衡及其在Sjogren综合征发病机制中的作用，特别是从病变局部T细胞免疫反应的角度。1我们从Sjogren综合征患者的唇活检获得的小涎腺中分离出唾液腺浸润T淋巴细胞，并进行特征性TH1或TH2细胞因子谱的检查。用抗CD3抗体刺激后细胞因子的体外产生测定以及从唾液组织分离的细胞的细胞内细胞因子分析证明了在Shogren综合征中T细胞浸润的TH1优势。对从唾液腺建立的许多T细胞克隆的进一步分析揭示了TH1细胞在组织中的积累。对患者外周血T细胞的类似研究表明，患有该疾病的个体具有全身免疫偏离以及局部TH1优势。2我们试图找到一种策略控制我们在该疾病中发现的免疫偏离的方法。体外实验表明，即使在体内系统中，TH1/TH2平衡的恢复也是可能的，本研究所用的方法将为分析与免疫病理抗原有关的自身免疫性疾病的TH1/TH2平衡和免疫偏离提供有用的应用。

This research project was aimed to analyze the TH1/TH2 imbalance and its role in the pathogenesis of Sjogren syndrome especially from the point of view of the local T cell immune responses at the lesion.1 We have isolated salivary glands-infiltrating T lymphocytes from minor salivary glands obtained by lip biopsy from the patients with Sjogren syndrome, and rendered to the examination for the characteristic TH1 or TH2 cytokine profile. The in vitro production assay of cytokines after stimulation with anti-CD3 antibodies as well as the intracellular cytokine analysis of the cells isolated from the salivary tissue demonstrated the TH1 predominance of the T cell infiltrates in Shogren's syndrome. Further analysis of the many T cell clones established from the salivary glands revealed the TH1 cells accumulation in the tissue. Similar studies with peripheral blood T cells from the patients indicated that the individuales with the disease have systemic immune deviation as well as the local TH1 predominance in the lesion.2 We attempted to find a way of strategic control of the immune deviation we found in the disease. In vitro administration of several compounds and cytokines showed the possible restoration of TH1/TH2 balance may be obtained even in the in vivo system.The approach we used in this study will provide a useful application to the analysis of TH1/TH2 balance and the immune deviation in the autoimmune diseases which are associated with immunopathological antigens most of that have not identified yet.