Relationship between p27^<Kip1> expression and tumor differention and prognosis in human oral squamous cell carcinoma.

我们用免疫组织化学方法检测了p27^&lt；kip1和gt；在口腔鳞状细胞癌中的表达，并探讨了各表达水平与口腔鳞癌的分级、T、N、分期、疗效及预后的关系。P27^&lt；Kip1和gt；高表达与高分化组(p&lt；0.05)、无组(p&lt；0.01)、非晚期组(p&lt；0.01)和有效组(p&lt；0.05)显著相关。高表达组的5年生存率为63.3%，显著高于低表达组的45.5%(p&lt；0.05)。根据Cox比例风险模型，p27、kip1、gt的高水平表达使死亡率显著降低。提示p27、kip1和gt；蛋白的表达可能与肿瘤的分级、转移抑制、疗效和预后密切相关，是一种有用的预后指标。接下来，我们用pcDNA3.1(Stratagene)构建了p27^；；Kip1&gt；表达载体。此外，我们还将其以正义或反义的方式导入口腔鳞状细胞癌细胞系(B88)，并试图在B88细胞中调控p27^；在B88细胞(B88/Tp27正义)中过表达p27^&lt；Kip1和Gt；抑制其生长、侵袭和转移。在B88细胞(B88/Tp27反义)中矛盾、低表达p27^&lt；Kip1和Gt；促进肿瘤的生长、侵袭和转移。在肿瘤分化方面，反义B88/Tp27可检测到由鳞状细胞型向梭形细胞型的形态变化，而HE染色检测不到亲本B88与B88/Tp27的差异。此外，免疫组织化学方法检测到B88/Tp27蛋白表达中有天山毛蛋白和角蛋白7，8(CaM 5.2)的表达。这些结果提示p27^&lt；Kip1&gt；可能与OSSC的终末分化有关。

We have investigated the expression of p27^<Kip1> in oral squamous cell carcinomas (OSCCs) using immunohistochemistry and have tried to investigate the relationship between each expression level and clinico-pathological characteristics such as tumor grade, T, N, stage classifications, effect and outcome. High levels of p27^<Kip1> expression were significantly related to well differentiated cases(p<0.05), NO cases (p<0.01), non advanced cases (p<0.01), and good effective cases (p<0.05). Five-year survival rate of p27^<Kip1> high expression cases was 63.3%, and it was significantly high than that of p27^<Kip1> low expression cases (45.5%) (p<0.05). By Cox's proportional hazards model, high levels of p27^<Kip1> expression significantly made the rate of death decrease. These results suggested that p27^<Kip1> protein expression may be closely related to tumor grade, inhibition of metastasis, effect and outcome, and an useful prognostic marker. Next, we have constructed p27^<Kip1> expression vectors with pcDNA3.1 (stratagene). Moreover, we transfected them to OSCC cell line (B88) in sense or anti-sense oriented, and tried to regulate the p27^<Kip1> gene in B88 cells. Overexpression of p27^<Kip1> in B88 cells (B88/Tp27sense) inhibited the growth, invasion and metastasis of them. Contradictory, low expression of p27^<Kip1> in B88 cells (B88/Tp27anti-sense) promoted the growth, invasion and metastasis. On tumor differentiation, we could detect the morphological change from squamous cell type to spindol cell type in B88/Tp27anti-sense though we could not detect the difference between parental B88 and B88/Tp27sense by HE staining. Moreover, we could detect the induction of involucrin and keratin 7,8 (Cam 5.2) in B88/Tp27sense immunohistochemically. These results suggested that p27^<Kip1> might be related to terminal differentiation of OSSC.