Algogenic auction of melittin ; bee venom

蜂毒素拍卖；

• 批准号: 11680807
• 负责人: KOYAMA Natsu
• 金额: $ 0.32万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680807/
• 关键词: melittin; pain; thermograph; axon reflex; sympathetic reflex; allodynia; PHN; rat; メリチンテスト; 痛み; 無髄C線維

Honeybee venom produces pain and accompanying inflammation. Bee venom contains many physiologically active substances ; i.e. apamin, melittin and others. The aim of the present study was to investigate whether melittin can be used in the study of pain in humans and rats. Intradermal melittin into the forearm in healthy humans temporally produced a severe pain and a subsequent skin temperature increase monitored by thermograph. Topical application of lidocaine inhibited the temperature increase. Patients with postherpetic neuralgia (PHN) were classified into 2 subgroups. In allodynia group, intradermal melittin into the PHN skin produced a significant pain, although less marked than in the normal skin. Melittin-induced temperature increase in the PHN skin was lower than in the normal skin. In non-allodynia group, intradermal melittin injection into the PHN skin produced little pain and did not elicit any significant temperature increase. These data suggest that C fibers are decreased in … More the PHN skin. This melittin test can be used to assess impairment of C fibers in PHN.The intradermal melittin into the forearm decreased the skin temperature of both palms immediately after the injection, and then increased well above the control level prior to the injection. The duration of the initial decrease was comparable to the duration of pain sensation. The initial decrease was interpreted as sympathetic vasoconstrictor reflex, and the later increase as inhibition of sympathetic vasomotor activity induced by noxious stimulation.Subcutaneous melittin into the hindpaw of pentobarbital-anesthetized rat induced a local skin temperature increase. Subcutaneous bee venom produced a comparable skin temperature increase, but apamin didn't. Melittin injected into the L4 or L5 dorsal root ganglion or sciatic nerve increased the skin temperature. These data suggest that melittin is the algogenic substance and that the melittin-induced skin temperature increase involves neurogenic mechanisms including axon reflexes and dorsal root reflexes. Less

蜜蜂毒液产生疼痛和伴随的炎症。蜂毒含有许多生理活性物质，如蜂毒肽、蜂毒肽等。本研究的目的是调查蜂毒素是否可以用于人类和大鼠疼痛的研究。皮内蜂毒肽到健康人前臂暂时产生了严重的疼痛和随后的皮肤温度升高监测温度计。局部应用利多卡因可抑制体温升高。带状疱疹后遗神经痛（PHN）患者分为2个亚组。在异常性疼痛组，皮内注射蜂毒肽到PHN皮肤产生了显著的疼痛，但比正常皮肤中的疼痛要轻。蜂毒肽引起的PHN皮肤温度升高低于正常皮肤。在非异常性疼痛组中，皮内注射蜂毒肽到PHN皮肤产生轻微疼痛，并且没有引起任何显著的温度升高。这些数据表明，C纤维减少， ...更多信息 PHN皮肤蜂毒肽试验可用于评估PHN中C纤维的损伤。前臂皮内注射蜂毒肽后立即降低双手掌的皮肤温度，然后在注射前升高至远高于对照水平。初始降低的持续时间与疼痛感觉的持续时间相当。在戊巴比妥麻醉的大鼠后爪皮下注射蜂毒肽，可引起局部皮肤温度升高。皮下蜂毒产生了类似的皮肤温度增加，但蜂毒没有。L4或L5背根神经节或坐骨神经注射蜂毒肽可使皮肤温度升高。这些数据表明，蜂毒肽是致痛物质，蜂毒肽诱导的皮肤温度升高涉及神经原性机制，包括轴突反射和背根反射。少

项目成果

Koyama N. et al: "Computor-assisted infrared thermographic study of axon reflex induced by intradermal melittin"PAIN. 84(2,3). 133-139 (2000)

Koyama N. 等人：“皮内蜂毒肽诱导的轴突反射的计算机辅助红外热成像研究”PAIN。

小山なつ,横田敏勝: "神経成長因子と痛み"日本ペインクリニック学会誌. 7(2). 103-115 (2000)

Natsu Koyama，Toshikatsu Yokota：“神经生长因子与疼痛”日本疼痛临床医师学会杂志 7(2) (2000)。

Hirata K, Higa K: "Herpes zoster. (in Japanese)"Rinsyou & Kenkyu. 76. 1915-1919 (1999)

平田 K、比嘉 K：“带状疱疹。（日语）”Rinsyou

Hirata K, Higa K: "Herpes zoster. (in Japanese)"Pain clinic. 20. S40-S46 (1999)

Hirata K、Higa K：“带状疱疹。（日语）”疼痛诊所。

Hirata K, Higa K: "Pain treatment of herpes zoster.(in Japanese)"Nippon-Rinsho. 58(4). 951-956 (2000)

Hirata K、Higa K：“带状疱疹的疼痛治疗。（日语）”Nippon-Rinsho。