マウス脳皮質形成過程の発現プロフィール解析

小鼠大脑皮层形成过程的表达谱分析

• 批准号: 12202032
• 负责人: 加藤 菊也
• 金额: --
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12202032/
• 关键词: 小脳皮質; 海馬歯状回; アダプター付加競合PCR法; laser capture microdissection

海馬歯状回は、小脳皮質と共通した性質をもっている。たとえば、小脳皮質の顆粒細胞を歯状回に移植すると歯状回型の顆粒細胞に分化する。両者の形成過程の遺伝子発現の類似性を想定し、発現プロファイルを比較検討した(なお、マウス小脳皮質の詳細な発現プロファイルについては昨年報告している)。約2000個の遺伝子の発現量をアダプター付加競合PCR法で生後2日、4日、12日、3週、6週の6点で測定を行い、クラスター分析を行った。小脳皮質の場合と同様十数個の限られた発現パターンに収斂した。それぞれの遺伝子の機能とパターンとの関係を調べてみると、小脳皮質の場合は、発生の初期に細胞増殖関連の遺伝子の発現が強く、後期に神経細胞の機能に携わる遺伝子の発現が強いという、顕著な傾向が認められた。歯状回の場合は発生の初期にproteasomeとリボゾーム蛋白が高発現する傾向がみられたが、それ以外では小脳皮質のような顕著な機能と発現パターンの連関は認められなかった。従って、この二つの層構造の形成過程は遺伝子発現の上では大きく異なることがわかった。また、上記の研究はすべて組織全体から精製したRNAを用いている。より詳細な発現プロフィール解析を行うため、まず層構造の明確な小脳皮質について、各層をLaser capture microdissectionで別々に採取して発現量を約500個の遺伝子についてアダプター付加競合PCR法で測定した。発生期の小脳は、外顆粒層、分子層、プルキンエ細胞層、内顆粒細胞層からなる。細胞分裂能がある顆粒細胞は外顆粒細胞層に、分化して分裂増殖能もたたないものは内顆粒細胞層に局在する。遺伝子の発現パターンは、だいたい解剖学的な所見と小脳全体の発現データからの推定と一致する。また、小脳全体の発現パターンは同じでも、LCMサンプルでは発現パターンのことなる遺伝子もかなり認められた。

The puffin-shaped gyrus and the small puffy skin have something in common with each other. The grafts were grafted in the shape of small skin, small skin, granulocytic cell, grafted gyrus, granulocytic differentiation. The cause of the process is that you want to determine the performance of the system, and that you are aware that there is a significant difference in the performance of the system. About 2000 tax dollars were added to the PCR method at 2: 00, 4: 00, 12: 00, 3: 00 and 6: 00 after birth. There are more than a dozen limited companies in cooperation with each other in order to meet the needs of the public. In the early stage of life, the growth of the baby is strong, and in the later period, the machine can carry the baby to make sure that the baby is strong, and in the later period, the machine can carry the machine to make sure that the baby is strong, and in the later period, the machine can carry the machine to make sure that the baby is strong, and that the baby can be used in the later period. In the early days of the health cycle, the high level of protein in the proteasome cell can be used to detect the number of microphones that can be linked to each other. During the whole process, it is necessary to improve the performance of the students in the last two years. The whole body of the research organization is responsible for the use of RNA. In this way, you can use the PCR method to determine the value of each Laser capture microdissection, which is about 500 yuan per month. At the birth stage, there are small cells, outer grains, molecules, inner grains and inner cells. The cell division can be divided into two parts: the outer cell, the outer cell, the differentiation cell, the division cell, the cell. In the study of anatomy, the whole body of anatomy showed that it was presumed to be consistent. We all know that we are in the same boat, and that we all know that we are in the same situation, and that we all know that we are in the same situation, and that we all know that we are in the same situation, and that we all know that we are in the same situation, and that we all know that we are in the same situation, and that we all know that we are in the same boat, and that we all know that we are in the same situation, and that we all know that we are in the same boat, and that we all know that we are in the same boat, and that we all know that we are in the same boat, and that LCM is not the same as we are.

项目成果

Kato,Kikuya: "Adaptor-tagged competitive PCR and its application to the mammalian nervous system"Comptes rendus de l' Academie des sciences. (in press).

Kato, Kikuya：“适配器标记的竞争性 PCR 及其在哺乳动物神经系统中的应用”Comptes rendus de l Academie des Sciences。

松原謙一: "発現プロファイルとトランスクリプトーム"中山書店(寄与分32頁). (2000)

Kenichi Matsubara：“表达谱和转录组”中山书店（贡献 32 页）（2000 年）。