Gene Detection by Light-to-electric Converting Intercalator and Its Application to DNA

光电转换嵌入剂基因检测及其在DNA中的应用

基本信息

  • 批准号:
    12305054
  • 负责人:
  • 金额:
    $ 15.33万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

In this research we developed a novel detection system of DNA duplex using a light-to-electric conversion. The principle of the detection of DNA duplex formation is as follows. 1) The gold electrode modified by single stranded DNA was prepared. 2) When double stranded DNA is formed, ruthenium complex will intercalate and will be concentrated on the electrode surface. 3) Then the visible light is irradiated to the electrode, photocurrent caused by excitation of ruthenium complex will be observed.We used a polypyridyl ruthenium complex carrying DNA intercalating ligand for DNA detection. Ru(bpy)_3 was used as a control compound. Ru(bpy)_2dppz was used as a ruthenium complex carrying a DNA intercalating part. Furthermore, we synthesized NDI-Ru_2 as a threading intercalator carrying ruthenium complex. This type of compound has a smaller dissociation rate from DNA duplex, because it has threading intercalating part connected to two bulky groupsA DNA-immobilized gold electrode was dipped into the solution containing the light-to-electric converting intercalator (Ru(bpy)_3, Ru(bpy)_2dppz, NDI-Ru_2). When the visible light was irradiated to the surface of the electrode, continuous photocurrent occurred in the presence of tertiary amine as an electron donor. The amount of photocurrent increased by 36% in the case of Ru(bpy)_2dppz, 86% in the case of NDI-Ru_2 after the formation of double-stranded DNA. This result suggested that the metallointercalator was concentrated on the surface of gold electrode by its affinity to DNA duplex. In the case of NDI-Ru_2, we consider that electron was smoothly transferred from ruthenium to gold electrode through naphthalenediimide because NDI acts as an electron acceptor. In conclusion, we could discriminate between double stranded and single stranded DNA from the magnitude of the photocurrent using a ruthenium intercalator.
在这项研究中,我们开发了一种新颖的利用光电转换的DNA双链检测系统。检测DNA双链形成的原理如下。1)制备了单链DNA修饰的金电极。2)当形成双链DNA时,Ru的络合物会嵌入并集中在电极表面。3)将可见光照射到电极上,观察到Ru配合物激发产生的光电流。我们使用了一种带有DNA插层配体的多吡啶Ru配合物来检测DNA。以Ru(Bpy)3为对照化合物。以Ru(Bpy)_2dppz作为Ru(Bpy)_2dppz嵌入DNA的配合物。此外,我们还合成了NdI-Ru_2作为负载Ru络合物的线状插层剂。将DNA固定的金电极浸泡在含有光电转换剂(Ru(Bpy)_3,Ru(Bpy)_2dppz,NdI-Ru_2)的溶液中。当可见光照射到电极表面时,在叔胺作为电子供体的存在下,产生了连续的光电流。在形成双链DNA后,Ru(Bpy)_2dppz的光电流增加了36%,NDI-Ru_2的光电流量增加了86%。这一结果表明,金属嵌入剂是通过与DNA双链的亲和力集中在金电极表面的。在NdI-Ru_2的情况下,我们认为,由于NdI作为电子受体,电子通过萘二亚胺顺利地从Ru转移到金电极上。综上所述,我们可以通过使用一台Ru嵌入器的光电流的大小来区分双链和单链DNA。

项目成果

期刊论文数量(27)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.Takagi: "Threading intercalation to double-stranded DNA and the application to DNA sensing. Electrochemical array technique"Pure Appl. Chem.. 73・10. 1573-1577 (2001)
M.Takagi:“双链 DNA 的穿线插入及其在 DNA 传感中的应用。电化学阵列技术”Pure Appl. 73・1577 (2001)
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    0
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S.Takenaka: "Small Molecule DNA and RNA Binders : From Synthesis to Nucleic Acid"Wiley-VCH. 23 (2003)
S.Takenaka:“小分子 DNA 和 RNA 结合剂:从合成到核酸”Wiley-VCH。
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    0
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A.Kumasaki, et al.: "A cyclic sugar unit interacts more strongly with DNA than do open-chain polyol coun erparts in sugar-linked synthetic DNA intercalators"Chemistry Letters. 2001. 370-371 (2001)
A.Kumasaki 等人:“在糖连接的合成 DNA 嵌入剂中,环状糖单元与 DNA 的相互作用比开链多元醇对应部分更强”。
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
K.Yamashita, M.Takagi, H.Kondo, S.Takenaka: "Electrochemical detection of nucleic base mismatches with ferrocenyl naphthalene diimide"Analytical Biochemistry. Vol.306. 188-196 (2002)
K.Yamashita、M.Takagi、H.Kondo、S.Takenaka:“用二茂铁基萘二酰亚胺电化学检测核酸碱基错配”分析生物化学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
A.Kumasaki, et al.: "A cyclic sugar unit interacts more strongly with DNA than do open-chain polyol counterparts in sugar-linked synthetic DNA intercalators"Chemistry Letters. 370-371 (2001)
A.Kumasaki 等人:“在糖连接的合成 DNA 嵌入剂中,环状糖单元与 DNA 的相互作用比开链多元醇对应物更强”,《化学快报》。
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    0
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TAKAGI Makoto其他文献

TAKAGI Makoto的其他文献

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{{ truncateString('TAKAGI Makoto', 18)}}的其他基金

Nanofabrication and Strengthening of Silicon Single Crystal by UsingSPM
利用SPM对硅单晶进行纳米加工和强化
  • 批准号:
    22560124
  • 财政年份:
    2010
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Preparation of Tribological Materials for Microelectromechanical systems
微机电系统摩擦学材料的制备
  • 批准号:
    13650766
  • 财政年份:
    2001
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
High-throughput Screening of Proteins by Electrochemical Protein Chip
电化学蛋白质芯片高通量筛选蛋白质
  • 批准号:
    12555236
  • 财政年份:
    2000
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Tribology of Nanostructured Materials for the Application to the Micromachine
纳米结构材料摩擦学在微机械中的应用
  • 批准号:
    11650726
  • 财政年份:
    1999
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Establishment of Fundamental Technique of Electrode Surface Modification for Development on Molecularly Imprinted Sensor
分子印迹传感器开发电极表面修饰基础技术的建立
  • 批准号:
    09555265
  • 财政年份:
    1997
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Novel Molecular Imprinting by Latex Polymerization-Basic Research for the Structure of Resin Surface-
乳胶聚合新型分子印迹-树脂表面结构的基础研究-
  • 批准号:
    07405038
  • 财政年份:
    1995
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of Novel Metal Ion Selective Ion-exchange Resin Based on Surface Template Polymerization
基于表面模板聚合的新型金属离子选择性离子交换树脂的研制
  • 批准号:
    06555256
  • 财政年份:
    1994
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
有機電解質の薄膜を用いる交流検出型気体センサの研究
有机电解质薄膜交流检测型气体传感器的研究
  • 批准号:
    01850172
  • 财政年份:
    1988
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B).
Development of ion-permselective piezodialysis membranes
离子选择性渗透压电渗析膜的开发
  • 批准号:
    60470066
  • 财政年份:
    1985
  • 资助金额:
    $ 15.33万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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