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Novel Molecular Imprinting by Latex Polymerization-Basic Research for the Structure of Resin Surface-

乳胶聚合新型分子印迹-树脂表面结构的基础研究-

基本信息

批准号：
07405038
负责人：
TAKAGI Makoto
金额：
$ 13.06万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1998
项目状态：
已结题

项目摘要

In recent years, the research area of design and synthesis of artificial host molecules is expanding focused by molecular recognition. We have developed a new method of molecular imprinting which produce a recognition site on the surface of latex particle.We started with a basic investigation of a system without template to connect the condition of emulsion and the structure of latex surface. The broadening of pKa was observed from proton titration of the resin. This result suggests the strong interaction between the functional groups and it is evidence that the carboxyl groups exist two dimensionally.We chose amino acid-derivatives as cationic organic template and synthesized imprinted resin. L-Phe-imprinted resin had high ability for adsorption of L-Phe. This result suggests the specific recognition site for L-Phe on the surface of the resin.We had used oleic acid as a functional surfactant, but there was possibility of missing of oleic acid from the resin. Then we designed and synthesized a new functional monomer possessing vinyl group. The form and character of the new resin was obviously different from the case of oleic acid. It is unknown what occurs during the surfactant was polymerized in the emulsion, but it was an interesting phenomenon.We tried an application to the membrane electrode-type ion sensor as a method for evaluation of imprinting effect. Molecular imprinted membrane was prepared by the polymerization after permeation of the salt of-functional monomer and organic cation into the membrane. This ion sensing membrane effectively responded to the templated ion, but it was not perfect Nernstian response.

近年来，以分子识别为中心的人工宿主分子的设计与合成研究领域不断扩大。我们发展了一种新的分子印迹方法，即在乳胶粒表面产生一个识别位点，我们从一个无模板体系的基础研究开始，将乳液条件和乳胶粒表面结构联系起来。从树脂的质子滴定中观察到pKa的加宽。这一结果表明了功能基团之间的强相互作用，证明了羧基是二维存在的。我们选择氨基酸衍生物作为阳离子有机模板，合成了印迹树脂。L-苯丙氨酸印迹树脂对L-苯丙氨酸有较强的吸附能力。这一结果表明，L-Phe在树脂表面上具有特异性识别位点，我们曾使用油酸作为功能表面活性剂，但存在油酸从树脂上丢失的可能性。然后设计合成了一种新的含乙烯基的功能单体。新树脂的形态和性质明显不同于油酸树脂。表面活性剂在乳液中聚合的过程中发生了什么尚不清楚，但这是一个有趣的现象。我们尝试将其应用于膜电极型离子传感器，作为评价印迹效果的方法。将功能单体的盐和有机阳离子渗透到膜中，通过聚合反应制备分子印迹膜。该离子传感膜有效地响应于模板离子，但它不是完美的能斯特响应。