Establishment of Fundamental Technique of Electrode Surface Modification for Development on Molecularly Imprinted Sensor

分子印迹传感器开发电极表面修饰基础技术的建立

• 批准号: 09555265
• 负责人: TAKAGI Makoto
• 金额: $ 7.49万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09555265/
• 关键词: molecular imprinting; sensor; gold electrode; molecular recognition; polymer membrane

In recent years, sensor for selective detection of target molecular is developed. We have developed a new method of molecular imprinting, and have been trying to apply the method to sensor.We started with a basic investigation of the structure of molecularly imprinted resin surface. The broadening of pKa was observed from proton titration of the resin. This results suggests the strong interaction between the functional groups and it is evidence that the carboxyl groups exist two dimensionally.Formation of Self-assembled monolayer from alkaneselenol and electrochemical characterization of the modified electrode were investigated. Alkaneselenol absorb to gold electrode more strongly than corresponding alkanethiol. It should be understood from the fact that selenium is a softer ligand than sulfur and has high affinity to class B metal such as gold and silver.We tried an application to the membrane electrode-type ion sensor as a method for evaluation of imprinting effect. Molecular imprinting membrane was prepared by the polymerization after permeation of the salt of functional monomer and organic cation into the membrane. This ion sensing membrane effectively responded to the template ion, but it was not perfect Nernstian response.

近几年发展起来的目标分子选择性检测传感器。我们开发了一种新的分子印迹方法，并一直在尝试将该方法应用于传感器。我们从分子印迹树脂表面结构的基础研究开始。通过树脂的质子滴定观察到pKa的增宽。这一结果表明官能团之间存在很强的相互作用，证明了羧基是二维存在的。研究了烷基硒醇自组装单分子膜的形成及其修饰电极的电化学性能。烷基硒醇比相应的烷基硫醇在金电极上的吸附更强。应该理解的是，硒是一种比硫更软的配体，对金和银等B类金属具有很高的亲和力。我们尝试将其应用于膜电极型离子传感器，作为评价印迹效果的一种方法。将功能单体的盐和有机阳离子渗透到膜中，通过聚合反应制得分子印迹膜。该离子敏感膜对模板离子有较好的响应，但不是理想的能斯特响应。

项目成果

S.Nakamura: "Molecularly Imprinted Resin for Recognition of Amino Acids Using Self-Assembly at o/w Interface"Transaction of Materials Research Society of Japan. 24(3). 453-456 (1999)

S.Nakamura：“利用 o/w 界面自组装来识别氨基酸的分子印迹树脂”日本材料研究学会汇刊。

K. Nakano: "DNA-Modified Electrode. Molecular Recognition of DNA and Biosensor Applications"A. G. Volkov Ed, "Liquid Interfaces in Chemical, Biological, and Pharmaceutical applications", Marcel Dekker, New York. (印刷中).

K. Nakano：“DNA 修饰电极。DNA 的分子识别和生物传感器应用”A. G. Volkov Ed，“化学、生物和制药应用中的液体界面”，Marcel Dekker，纽约（出版中）。

中野 幸二: "自己組織化単分子膜による化学修飾電極" 日本接着学会誌. 33・6. 240-247 (1997)

Koji Nakano：“使用自组装单层的化学修饰电极”日本粘附协会杂志 33・6（1997）。

Y. Koide: "Adsorption of Metal Ions to Surface-Template Resins Prepared with Amphiphilic Styrene Monomers Bearing Amino Carboxylic Acid"Bull. Chem. Soc. Jpn.. 71. 789-796 (1998)

Y. Koide：“金属离子对用带有氨基羧酸的两亲性苯乙烯单体制备的表面模板树脂的吸附”Bull。

K. Tsukagoshi et al.: "Surface Imprinting : Preparation of Metal Ion-Imprinting Resins by Use of Complexation at the Aqueous-Organic Interface"ACS SYMPOSIUM SERIES. 703. 251-263 (1998)

K. Tsukagoshi 等人：“表面压印：通过在水-有机界面络合制备金属离子压印树脂”ACS 研讨会系列。