Establishment of Fundamental Technique of Electrode Surface Modification for Development on Molecularly Imprinted Sensor

近年来，用于选择性检测靶分子的传感器得到了发展。我们开发了一种新的分子印迹方法，并尝试将其应用于传感器。我们从分子印迹树脂表面结构的基本研究开始。通过质子滴定法观察到pKa的展宽。这一结果表明，官能团之间存在很强的相互作用，并证明羧基是二维存在的。研究了烷烯醇自组装单层电极的形成及其电化学表征。烷烯醇对金电极的吸附比相应的烷硫醇更强。硒是一种比硫更软的配体，对金、银等B类金属具有较高的亲和力。我们尝试将其应用于膜电极型离子传感器，作为印迹效果评价的方法。将功能单体盐和有机阳离子渗透到膜中，通过聚合法制备分子印迹膜。该离子传感膜对模板离子有较好的响应，但不是完全的能斯汀响应。

In recent years, sensor for selective detection of target molecular is developed. We have developed a new method of molecular imprinting, and have been trying to apply the method to sensor.We started with a basic investigation of the structure of molecularly imprinted resin surface. The broadening of pKa was observed from proton titration of the resin. This results suggests the strong interaction between the functional groups and it is evidence that the carboxyl groups exist two dimensionally.Formation of Self-assembled monolayer from alkaneselenol and electrochemical characterization of the modified electrode were investigated. Alkaneselenol absorb to gold electrode more strongly than corresponding alkanethiol. It should be understood from the fact that selenium is a softer ligand than sulfur and has high affinity to class B metal such as gold and silver.We tried an application to the membrane electrode-type ion sensor as a method for evaluation of imprinting effect. Molecular imprinting membrane was prepared by the polymerization after permeation of the salt of functional monomer and organic cation into the membrane. This ion sensing membrane effectively responded to the template ion, but it was not perfect Nernstian response.