Establishment of Fundamental Technique of Electrode Surface Modification for Development on Molecularly Imprinted Sensor

近年来，开发了用于目标分子的选择性检测的传感器。我们发展了一种新的分子印迹方法，并尝试将其应用于传感器。从树脂的质子滴定中观察到pKa的加宽。这一结果表明，官能团之间存在较强的相互作用，证明了羧基是二维存在的。研究了烷硒醇自组装膜的形成及修饰电极的电化学性能。烷硒醇在金电极上的吸附比相应的烷硫醇强。由于硒是比硫更软的配体，与金、银等B类金属的亲和性高，因此作为印迹效果的评价方法，尝试应用于膜电极型离子传感器。将功能单体与有机阳离子的盐渗透到膜中，通过聚合反应制备分子印迹膜。该离子敏感膜对模板离子有较好的响应，但不是理想的能斯特响应。

In recent years, sensor for selective detection of target molecular is developed. We have developed a new method of molecular imprinting, and have been trying to apply the method to sensor.We started with a basic investigation of the structure of molecularly imprinted resin surface. The broadening of pKa was observed from proton titration of the resin. This results suggests the strong interaction between the functional groups and it is evidence that the carboxyl groups exist two dimensionally.Formation of Self-assembled monolayer from alkaneselenol and electrochemical characterization of the modified electrode were investigated. Alkaneselenol absorb to gold electrode more strongly than corresponding alkanethiol. It should be understood from the fact that selenium is a softer ligand than sulfur and has high affinity to class B metal such as gold and silver.We tried an application to the membrane electrode-type ion sensor as a method for evaluation of imprinting effect. Molecular imprinting membrane was prepared by the polymerization after permeation of the salt of functional monomer and organic cation into the membrane. This ion sensing membrane effectively responded to the template ion, but it was not perfect Nernstian response.