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Fundamental study on the mechanisms and treatments for paresthesia and dysesthesia following traumatic injury of trigeminal nerve

三叉神经损伤后感觉异常、感觉迟钝的机制及治疗基础研究

基本信息

批准号：
12671930
负责人：
SEO Kenji
金额：
$ 2.24万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671930/
关键词：
TRIGEMINAL CAUDALIS OPTICAL IMAGING SLICE RH414 CNQX MK-801 NK1-RECEPTOR Trigeminal Subnucleus caudalis 膜電位画像蛍光色素 GABA グルタミン酸 rat

项目摘要

It is well known that traumatic injury of trigeminal nerve sometimes follows a long time paresthesia and dysesthesia and we hypothesized that these symptoms might come from the long-term excitation of trigeminal nucleus. Therefore, using high-speed optical imaging technique, we observed the propagation of neuronal excitation in the nociceptive afferent transmission and modulating site the trigeminal subnucleus caudalis (Vc), which was evoked by stimulation of primary afferent fibers.Medulla was removed from mice or rats, and coronal, sagittal or horizontal sectioned slices of 400 μm thicknesses were stained with a voltage-sensitive fluorescence dye RH414. Fluorescence and differential fluorescence images of a slice preparation were recorded using a high-speed imaging system. The tract of each slice was stimulated directly by single shock or a train of 30 pulses at 100 s^<-1>. Either in the sagittal or coronal slices, membrane excitation evoked by single stimulation of the tract did not expand in any directions. Larger propagations of evoked by the single stimulation to the marginal layer or substantia gelatinosa were observed in the sagittal rather than coronal slices. In contract, in the horizontal slices, propagations of membrane excitation, which were evoked by single stimulation, expanded from the Marginal layer (Mar) to the substantia gelatinosa (SG). And an exposure to CNQX suppressed these propagations. High frequency stimulation induced widely propagated and long-lasting membrane depolarizations within Mar, Mg and Magnocellularis area (Mar). Exposure to MK-801 suppressed the depolarization expansion to SG.These results suggest that horizontal slice is suitable for the study on the excitation propagation evoked by the afferent fiber stimulation within the trigeminal caudalis. Moreover, these results also support that glutamate might also be a neurotransmitter within the trigeminal afferent pathway in Vc.

众所周知，外伤性三叉神经损伤有时会出现长时间的感觉异常和感觉不良，我们推测这些症状可能来自三叉神经核的长期兴奋。因此，我们利用高速光学成像技术，观察了三叉神经尾侧亚核（Vc）痛觉传入神经传递和调节部位神经元兴奋的传播，该神经元兴奋是由初级传入神经纤维刺激引起的。取小鼠或大鼠髓质，用电压敏感荧光染料RH414对400 μm厚度的冠状、矢状或水平切片进行染色。利用高速成像系统记录切片制备的荧光和差示荧光图像。每个切片的束直接受到单次冲击或30次脉冲序列的100 s^<-1>的刺激。无论是矢状面还是冠状面，单次刺激引起的膜兴奋均未向任何方向扩张。在矢状面而不是冠状面，观察到单次刺激对边缘层或明胶质引起的更大的传播。在水平片上，单次刺激引起的膜兴奋的传播从边缘层（Mar）扩展到明胶质（SG）。暴露于CNQX中会抑制这些传播。高频刺激在Mar、Mg和manocellularis区（Mar）内诱导了广泛传播和持久的膜去极化。暴露于MK-801抑制退极化扩展到SG。这些结果表明，水平切片适合于研究传入纤维刺激在三叉神经尾侧引起的兴奋传播。此外，这些结果也支持谷氨酸也可能是Vc三叉神经传入通路中的神经递质。