Rhythm generation in honeybees: feeding cycle and period

蜜蜂节律的产生：进食周期和周期

• 批准号: 13640679
• 负责人: SHIMIZU Isamu
• 金额: $ 0.64万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640679/
• 关键词: HONEY BEE; CIRCADIAN RYTHM; IN SITU HYBRIDYDAZATION; BIOLOGICAL CLOCK; PERIOD GENE; FEEDING CYCLE; BIODERVERSITY; SPLICED VARIANTS; インサイチューハイブリ; ニホンミツバチ; サーカデイアンリズム; 明暗サイクル; 自由継続リズム; 概日性リズム; バイオトロン

In this project structure and expression of period gene in honeybees, Apis cerana japonica were investigated to reveal the molecular mechanism of temporal division of behavioral activity in the field, which is considered to be one of the basis of biodiversity maintenance in the multi-species coexistence. At first the characteristics of circadian rhythm in the honeybee were investigated using individual workers and a colony that was entrained in Zootron. Then period gene encoding cDNA was cloned by use of PCR strategy, and found fours kinds of spliced alternative variants exist. To investigate the molecular mechanism of formation of period m-RNA variants, genomic structure of the honeybee was analysed on the encoding period gene region including introns. Further RT-PCR experiments showed tissue specific expression patterns in worker bees. The results obtained suggested that there are some slave peripheral clocks in addition to the main clock localized in the brain.

在这种项目结构和蜜蜂中周期基因的表达中，研究了API Cerana Japonica，以揭示该田间行为活性时间分裂的分子机制，该田间行为活性的分子机制，这被认为是多种物种共存中生物多样性维持的基础之一。首先，使用单个工人和一个夹杂在Zootron的殖民地研究了蜜蜂中昼夜节律的特征。然后，通过使用PCR策略克隆了编码cDNA的周期基因，并发现存在四种剪接替代变体。为了研究周期M-RNA变体形成的分子机制，在包括内含子（内含子）的编码期基因区域分析了蜜蜂的基因组结构。进一步的RT-PCR实验显示了工人蜜蜂中组织特异性表达模式。获得的结果表明，除了大脑中的主钟外，还有一些从奴隶外围钟。

项目成果

清水勇, 源利文: "見える世界が魚を変える-魚類の多様性と視覚適応(生物多様性科学のすすめ-生態学からのアプローチ:大串隆之編)"丸善株式会社. 186 (2003)

Isamu Shimizu、Toshifumi Minamoto：“可见的世界改变了鱼类 - 鱼类的多样性和视觉适应（促进生物多样性科学 - 来自生态学的方法：由 Takayuki Ohkushi 编辑）” Maruzen Co., Ltd. 186 (2003)

Takami Y., Shimizu I.et al.: "Genetic diversity and structure of urban populations of Pieris butterflies assessed using amplified fragment length polymorphism"Mol.Ecol.. 13. 245-258 (2004)

Takami Y.、Shimizu I.等人：“使用扩增片段长度多态性评估菜粉蝶城市种群的遗传多样性和结构”Mol.Ecol.. 13. 245-258 (2004)

Minamoto T., Shimizu I.: "Studies of opsin genes in a smelt fish, Ayu (Plecoglosssus altivelis)"J. Photoscience. 9. 269-271 (2002)

Minamoto T.，Shimizu I.：“胡瓜鱼视蛋白基因的研究，Ayu (Plecoglossus altivelis)”J.

Shimizu I., Yamakawa Y., et al.: "Molecular cloning of Bombyx cerebral opsin (Boceropsin) and cellular localization of its expression in the silkworm brain."Biochem.Biophys.Res.Commun.. 287. 27-34 (2001)

Shimizu I., Yamakawa Y., et al.：“家蚕脑视蛋白 (Boceropsin) 的分子克隆及其在蚕脑中表达的细胞定位。”Biochem.Biophys.Res.Commun.. 287. 27-34 (2001)

Shimizu I., Kawai Y., et al.: "Circadian rhythm and cDNA cloning of the clock gene period in the honeybee Apis cerana japonica"Zool Science. 18. 779-789 (2001)

Shimizu I.、Kawai Y.等人：“蜜蜂 Apis cerana japonica 的昼夜节律和时钟基因周期的 cDNA 克隆”《动物科学》。