Rhythm generation in honeybees: feeding cycle and period

蜜蜂节律的产生：进食周期和周期

• 批准号: 13640679
• 负责人: SHIMIZU Isamu
• 金额: $ 0.64万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640679/
• 关键词: HONEY BEE; CIRCADIAN RYTHM; IN SITU HYBRIDYDAZATION; BIOLOGICAL CLOCK; PERIOD GENE; FEEDING CYCLE; BIODERVERSITY; SPLICED VARIANTS; インサイチューハイブリ; ニホンミツバチ; サーカデイアンリズム; 明暗サイクル; 自由継続リズム; 概日性リズム; バイオトロン

In this project structure and expression of period gene in honeybees, Apis cerana japonica were investigated to reveal the molecular mechanism of temporal division of behavioral activity in the field, which is considered to be one of the basis of biodiversity maintenance in the multi-species coexistence. At first the characteristics of circadian rhythm in the honeybee were investigated using individual workers and a colony that was entrained in Zootron. Then period gene encoding cDNA was cloned by use of PCR strategy, and found fours kinds of spliced alternative variants exist. To investigate the molecular mechanism of formation of period m-RNA variants, genomic structure of the honeybee was analysed on the encoding period gene region including introns. Further RT-PCR experiments showed tissue specific expression patterns in worker bees. The results obtained suggested that there are some slave peripheral clocks in addition to the main clock localized in the brain.

本课题通过对中华蜜蜂周期基因结构和表达的研究，揭示蜜蜂田间行为活动时序划分的分子机制，为多物种共存环境下维持生物多样性提供依据。首先，利用个体工蜂和携带在Zootron中的蜂群研究了蜜蜂的昼夜节律特征。然后利用聚合酶链式反应技术克隆了周期基因，发现存在4种剪接变异体。为了探讨周期m-RNA变异形成的分子机制，在包括内含子在内的周期基因编码区分析了蜜蜂的基因组结构。进一步的RT-PCR实验显示了工蜂的组织特异性表达模式。这些结果表明，除了定位于大脑的主时钟外，还有一些从属的外周时钟。

项目成果

清水勇, 源利文: "見える世界が魚を変える-魚類の多様性と視覚適応(生物多様性科学のすすめ-生態学からのアプローチ:大串隆之編)"丸善株式会社. 186 (2003)

Isamu Shimizu、Toshifumi Minamoto：“可见的世界改变了鱼类 - 鱼类的多样性和视觉适应（促进生物多样性科学 - 来自生态学的方法：由 Takayuki Ohkushi 编辑）” Maruzen Co., Ltd. 186 (2003)

Takami Y., Shimizu I.et al.: "Genetic diversity and structure of urban populations of Pieris butterflies assessed using amplified fragment length polymorphism"Mol.Ecol.. 13. 245-258 (2004)

Takami Y.、Shimizu I.等人：“使用扩增片段长度多态性评估菜粉蝶城市种群的遗传多样性和结构”Mol.Ecol.. 13. 245-258 (2004)

Minamoto T., Shimizu I.: "Studies of opsin genes in a smelt fish, Ayu (Plecoglosssus altivelis)"J. Photoscience. 9. 269-271 (2002)

Minamoto T.，Shimizu I.：“胡瓜鱼视蛋白基因的研究，Ayu (Plecoglossus altivelis)”J.

Shimizu I., Yamakawa Y., et al.: "Molecular cloning of Bombyx cerebral opsin (Boceropsin) and cellular localization of its expression in the silkworm brain."Biochem.Biophys.Res.Commun.. 287. 27-34 (2001)

Shimizu I., Yamakawa Y., et al.：“家蚕脑视蛋白 (Boceropsin) 的分子克隆及其在蚕脑中表达的细胞定位。”Biochem.Biophys.Res.Commun.. 287. 27-34 (2001)

菅原道夫, 源利文, 清水勇, 東克: "キンリョウヘン Cymbidium floribundum唇弁の着色がニホンミツバチApis cerana japonicaの訪花行動にあたえる影響"ミツバチ科学. 24. 115-118 (2003)

Michio Sukawara、Toshifumi Minamoto、Isamu Shimizu、Katsu Higashi：“花蕙兰唇色对日本蜜蜂 Apis cerana japonica 访花行为的影响”《蜜蜂科学》24. 115-118 (2003)。