Investigation : on of insect Photoperidic receptor using moleculer Biology

研究：利用分子生物学研究昆虫光周受体

• 批准号: 08640861
• 负责人: SHIMIZU Isamu
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640861/
• 关键词: Insect; Photoperiodism; Photoreceptor; Opsin; Diapasue; Neure; Silkworm; 神経球; 光環境

The purpose of this project is an elucidation of the insect (silkworm) photoperiodic photoreceptor at the molecular level. We supposed the receptor which locates in the brain is rhodopsin-like molecule, and tried to reveal its structure and function by molecular biological techniques. In this project we found first that (1) the secretory site of diapause hormone is the complex of the corpora-allata and corpola-cardiaca complex, (2) and we identified the diapasue synthesising neurosecretory cells in the subesophageal complex. (3) We could amplify fragments using template of cDNA obtained from the compound eyes of the silkworm with primers designed on the basis of the conserved region of invertebrate opsin genes, and sequenced two fragements showing sequences which were similar to other opsins. (4) Finally we succeeded the amplification and subcloning one fragment of opsin-like pigment using cDNA of silkworm brain as template and the full length of the sequence was determined by 3'-and 5'-race method.

该项目的目的是在分子水平上阐明昆虫（蚕）的光周期光感受器。我们假设大脑中的受体是视紫红质样分子，并试图通过分子生物学技术揭示其结构和功能。在这个项目中，我们首先发现（1）滞育激素的分泌部位是体-下体和心体-贲门复合体的复合体，（2）我们在食管下复合体中鉴定了滞育合成神经分泌细胞。 (3)以家蚕复眼cDNA为模板，根据无脊椎动物视蛋白基因保守区设计引物，扩增片段，并对两个与其他视蛋白序列相似的片段进行测序。 (4)最后以蚕脑cDNA为模板，成功扩增并亚克隆了1个视蛋白样色素片段，并通过3'-和5'-race方法确定了该序列的全长。

项目成果

Shimizu, J.: "The effect of temperature on the tomporal strcture of the Uihratory countslup signals of a spicler." J.Comp.Physical (A). 179. 363-370 (1996)

Shimizu, J.：“温度对拼接器的 Uihratory 计数信号的颞结构的影响。”

Ichikawa, T.: "Functional differentation of neurosecretory cells with immunoreactive DH and PBAN of the Bombyx mori." Zoological Science. 13. 21-25 (1996)

Ichikawa, T.：“家蚕免疫反应性 DH 和 PBAN 神经分泌细胞的功能分化。”

Shimizu, I.: "Cloning of genes enioding the Uisual Pigments in the Silcuone" Appl.Entomol.Zool. 33. 199-204 (1998)

Shimizu, I.：“在 Silcuone 中克隆常见色素的基因”Appl.Entomol.Zool。

Shimizu, I.: "Neuroendocrine control of diapause hormone secretion in the silkworm, Bombyx mori." J.Insect Physiol. 43. 1101-1109 (1997)

Shimizu, I.：“家蚕滞育激素分泌的神经内分泌控制。”

I Shimizu and F.G.Berth: "The effect of temperature on the temporal structure of the vihratory ceistship signals of a spider der (cupienmius saley keys)" J.Comp.Physiol (A). 179. 363-370 (1996)

I Shimizu 和 F.G.Berth：“温度对蜘蛛 der (cupienmius saley keys) 振动信号时间结构的影响”J.Comp.Physiol (A)。