Physiological Research of Photoperiodic nesporse of insect using laser micro-beam.

激光微束对昆虫光周期孢子的生理研究。

• 批准号: 03640602
• 负责人: SHIMIZU Isamu
• 金额: $ 1.15万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640602/
• 关键词: laser-microbeam; Insect; Silkworm; diapause; diapause-hormone; neuro-sencation; phortoperiodism; 昆虫ホルモン; レ-ザ-メス

An apparatus which is devised to illuminate ultraviolet laser- microbeam to insect tissue and organ, and to examine its effect on the photoperiodic response of insects, was constructed. At first I tried an illumination of the laser-microbeam to subeophageal ganglion(SG) of the silkworm, in which the hormonal effector system is located. Brain(Br)-SG complex was extirpated from the head of pupae of diapause-egg producers, and setted on a glass- plate. The damage of the tissue was confirmed by an observation of some small bubbles which produced on the surface of the ganglions. When the beam was illuminated on the location of neurosecretory cells in SG the diapause percentage became 0%, while the control without illumination was 100%, it means that this laser-treatment was very effective as a micro-treatment technique. Regarding the illumination to brain of the silkworm, only preliminary results were obtained, but the destory of the some parts of protocerebral ganglion suggests that a central regulatory site might be located in this area.

设计了一种紫外激光微光束照射昆虫组织器官的装置，研究了其对昆虫光周期响应的影响。首先，我尝试用激光微束照射蚕的食道下神经节（SG），这是激素效应系统所在的部位。从滞卵蜂蛹的头部提取脑(Br)- sg复合物，置于玻璃板上。对神经节表面产生的一些小气泡的观察证实了组织的损伤。当光束照射在SG的神经分泌细胞位置时，滞育率为0%，而未照射的对照组滞育率为100%，这意味着激光治疗作为一种微治疗技术是非常有效的。关于家蚕脑的照明，目前只取得了初步的结果，但对原脑神经节部分部位的破坏表明，中枢调控位点可能位于该区域。

项目成果

Shimizu I.: "Regulation of diapause hormone senetion in the Silkworm,Bombyxmori:using laser microbeam treatment." Zoological Science. 9. 1271 (1992)

Shimizu I.：“家蚕滞育激素的调节：使用激光微束治疗。”

Shimizu.I.: "Comparison of tatly and comporition in lipids of diapanse and non-diapance eggs of Bombyx mori" Comp.Biochem.Physiol.102B. 713-716 (1992)

Shimizu.I.：“家蚕 diapanse 和非 diapanse 卵的脂质和组成的比较”Comp.Biochem.Physiol.102B。

AOKI S.: "Mechamism of syntkesis and secreation of diaqaese hormere in the silkwom,Bambyx mori" Zoological Science. 8. 1182-1183 (1991)

青木 S.：“家蚕中 diaqaese hormere 的合成和分泌机制，家蚕”动物学科学。

清水 勇: "カイコの化性と光周性反応" 応用動物昆虫学雑誌. 35. 83-91 (1991)

Isamu Shimizu：“蚕的成熟和光周期反应”应用动物昆虫学杂志 35. 83-91 (1991)。

SHIMIZU.I.: "Comqarison of fatty and comqorition in liqids of diaqanse and xm-diaqasees of Bomayx mori" Comq.Biochem.Physiol. 102B. 713-716 (1992)

SHIMIZU.I.：“家蚕 diaqanse 和 xm-diaqasees 液体中脂肪和 comqorition 的比较”Comq.Biochem.Physiol。