Ultrastructure and molecular phylogeny of CW and DO groups among unicellular green algae

单细胞绿藻CW和DO类群的超微结构和分子系统发育

• 批准号: 13640694
• 负责人: WATANABE Shin
• 金额: $ 1.28万
• 依托单位: Toyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640694/
• 关键词: green algae; Ultrastructure; Molecular Phylogeny; Taxonomy; Chlorococcales; Sphaelopleales; CW group; DO group; 国際情報交換

Results of CW group are described in 1 4, and those of DO group are in 5, as next. 1.Morphological and molecular phylogenetic analyses on 19 strains of sarcinoid-forming green algae revealed that Chlorosarcinopsis are divided into 4 subgroups, that Dcsmotetra is characterized by subapical basal bodies and parallel thylakoids in pyrenoids, that Chlorosarcina stigmatica(ASIB.T105) should be classified in a new genus, and that validity of Neochlorosarcina was ascertained. Physiological examinations on sarcinoid members by Groover and Bold(1969) was re-evaluated. 2.Heterotetracystis and Fasciculochloris bearing slightly unequal flagella are neither monophyletic to one another nor closely related with other unequal members, and the unequality was independently acquired. 3.Chlamydomonas monticola should be transferred to Heterotetrachalmydomonas by parallel basal bodies and unequal flagella, and is distinguished by naked gametes and anterior nucleus. 4.A unicellular green alga isolated from a farm soil of high salinity in Kazakhstan had chlorophyll a and b at the ratio of 2.62 in extraction of 80% aceton, and walled, astigmatic zoospores whose flagellar apparatus slightly bend from longitudinal axis of cell, and lengths of two flagella differ from one another at ratio of 2:1. This organism should be included in a new genus. 5.Phylogenetic analysis of Follicularia resolved a close affinity with Bracteacoccus in the Sphaeropleales. Despite of differences between them, the presence of numerous discoid chloroplasts are inferred to be their synapomorphic feature.

CW组结果见1～4，DO组结果见5。 1.对19株产生类肉瘤的绿藻进行形态学和分子系统发育分析，结果表明Chlorosarcinopsis可分为4个亚类，Dcsmotetra以近顶端基体和蛋白核中平行的类囊体为特征，Chlorosarcina stigmatica(ASIB.T105)应归类为新属， 确定了新绿八叠球菌的有效性。 Groover 和 Bold (1969) 对类肉瘤成员的生理检查进行了重新评估。 2.具有稍不等鞭毛的异四胞藻和束绿藻既不是单系的，也不与其他不等成员密切相关，不等是独立获得的。 3.衣藻应由平行基体和不等鞭毛向异四藻单胞菌转移，并以裸配子和前核区分。 4.从哈萨克斯坦高盐度农田土壤中分离出的一种单细胞绿藻，经80%丙酮提取后，叶绿素a和b的比例为2.62，游动孢子有壁、散光，鞭毛器从细胞纵轴略微弯曲，两根鞭毛长度相差2:1。该生物体应包含在一个新属中。 5.毛囊菌的系统发育分析表明其与球球菌目中的小球菌有密切的亲缘关系。尽管它们之间存在差异，但大量盘状叶绿体的存在被推断为它们的共形特征。