Ultrastructure and molecular phylogeny of CW and DO groups among unicellular green algae

CW组结果如下：14例，DO组5例，下一例。1.对19株形成肉瘤的绿藻进行了形态和分子系统发育分析，结果表明，绿藻可分为4个亚组，豆状绿藻具有亚尖基体和平行的类囊体特征，豆状绿藻应归为一个新属，并证实了新绿藻的有效性。重新评价了Groover和Bold(1969)对类肉瘤成员的生理检查。2.具有轻微不等鞭毛的异型四胞藻和绿曲霉既不是彼此的单系，也不与其他不等鞭类成员有密切的亲缘关系，这种不同是独立获得的。3.蒙氏衣藻应通过平行的基体和不等长的鞭毛转移到异形藻，并以裸露配子和前核为特征。4.从哈萨克斯坦高盐度农田土壤中分离到的一株单细胞绿藻，在80%丙酮提取液中，叶绿素a和b的比例为2.62，游动孢子有壁，鞭毛器略向细胞纵轴弯曲，两鞭毛长度之比为2：1，应归入一新属。5.系统发育分析表明，该菌与球孢菌目中的Bracteacocus有较近的亲缘关系。尽管它们之间存在差异，但大量盘状叶绿体的存在被推测为它们的突触特征。

Results of CW group are described in 1 4, and those of DO group are in 5, as next. 1.Morphological and molecular phylogenetic analyses on 19 strains of sarcinoid-forming green algae revealed that Chlorosarcinopsis are divided into 4 subgroups, that Dcsmotetra is characterized by subapical basal bodies and parallel thylakoids in pyrenoids, that Chlorosarcina stigmatica(ASIB.T105) should be classified in a new genus, and that validity of Neochlorosarcina was ascertained. Physiological examinations on sarcinoid members by Groover and Bold(1969) was re-evaluated. 2.Heterotetracystis and Fasciculochloris bearing slightly unequal flagella are neither monophyletic to one another nor closely related with other unequal members, and the unequality was independently acquired. 3.Chlamydomonas monticola should be transferred to Heterotetrachalmydomonas by parallel basal bodies and unequal flagella, and is distinguished by naked gametes and anterior nucleus. 4.A unicellular green alga isolated from a farm soil of high salinity in Kazakhstan had chlorophyll a and b at the ratio of 2.62 in extraction of 80% aceton, and walled, astigmatic zoospores whose flagellar apparatus slightly bend from longitudinal axis of cell, and lengths of two flagella differ from one another at ratio of 2:1. This organism should be included in a new genus. 5.Phylogenetic analysis of Follicularia resolved a close affinity with Bracteacoccus in the Sphaeropleales. Despite of differences between them, the presence of numerous discoid chloroplasts are inferred to be their synapomorphic feature.