Molecular mechanisms of the nerve net formation in hedra primitive nervous system

异体原始神经系统神经网络形成的分子机制

• 批准号: 13640687
• 负责人: KOIZUMI Osamu
• 金额: $ 0.7万
• 依托单位: Fukuoka Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640687/
• 关键词: diffuse nervous system; hydra; nerve net formation; developmental neurobiology; [peptide; immunohistochemistry; antibody production; nerve differentiation; 免疫組織化学; 抗体作成

(1)Large scale screening of peptide signal molecules : According to our peptide project, the following ideas were clarified.(i)Peptide molecules have developmental functions such as morphogenesis and cell differentiation in addition to the well known function, the transmission of neural signals.(ii)Peptide molecules are epithelial peptides localized in epithelial cells in addition to neuropeptides localized in nerve cells.(2)Chemical anatomy of the hydra nervous system using neuropeptide antibodies : We produced antibodies to all peptides which structures-were identified. Using these antibodies, we did chemical anatomy of hydra, and the detailed anatomical chart covering the whole body was able to be made.(3)Molecular mechanisms of the nerve net formation : We identified peptide molecules controlling the nerve differentiation. Hym355 is a neuropeptide activating the nerve differentiation. LPW peptides are epithelial peptides inhibiting the nerve differentiation.(4)Nerve cell culture system as a bia-assay system. We tried to establish the cell culture system of nerve cells. If it will be established, it will be used as a bioassay system to screen neuroactive substances or active substances influences on the nervous system. The present situation is a success of short term culture of single neurons.

(1)大规模筛选多肽信号分子：根据我们的多肽项目，阐明了以下观点：(I)多肽分子除了具有众所周知的神经信号传递功能外，还具有形态发生和细胞分化等发育功能。(Ii)多肽分子是定位于上皮细胞的上皮肽，也是定位于神经细胞的神经肽。(2)利用神经肽抗体对水蛇神经系统的化学解剖：我们针对所有结构已确定的多肽产生抗体。利用这些抗体对多头蛇进行了化学解剖，制作了覆盖全身的详细解剖图。(3)神经网络形成的分子机制：我们鉴定了控制神经分化的多肽分子。Hym355是一种激活神经分化的神经肽。LPW多肽是抑制神经分化的上皮肽。(4)神经细胞培养体系作为BIA检测系统。我们试图建立神经细胞的细胞培养体系。如果它将被建立，它将被用作一个生物测定系统来筛选神经活性物质或活性物质对神经系统的影响。目前的情况是短期培养单个神经元的成功。

项目成果

T.Takahashi: "A novel neuropeptide, Hym355, positively regulates neuron differentiation In hydra"Development. 127. 997-1005 (2000)

T.Takahashi：“一种新型神经肽，Hym355，正向调节水螅中的神经元分化”的开发。

T.Takahashi: "Identification of a new member of the GLWamide peptide family : physiological activity and cellular localization in cnidarian polyps."Comp.Biochem.Physiol.. 135B. 309-324 (2003)

T.Takahashi：“GLWamide 肽家族新成员的鉴定：刺胞动物息肉中的生理活性和细胞定位。”Comp.Biochem.Physiol.. 135B。

D.Asai: "Biochemical evaluation of hormonal activity of endocrine disruptors by sensing the estrogen receptor conformation changes"Biol. Bull. (in press). (2003)

D.Asai：“通过感知雌激素受体构象变化对内分泌干扰物的激素活性进行生化评估”Biol。

S.Shimizu: "The diffuse nerve net in Hydra body column represents a primitive enteric nervous system."J.comp.physiol.. in Press. (2004)

S.Shimizu：“水螅体柱中的弥漫神经网络代表了原始的肠神经系统。”J.comp.physiol.. 出版。

O.Koizumi: "Developmental neurobiology of hydra, a model animal of cnidarians"Canadian Journal of Zoology. 80. 1678-1689 (2002)

O.Koizumi：“刺胞动物模型动物水螅的发育神经生物学”加拿大动物学杂志。