DEVELOPMENT OF THE LASER ULTRASONIC MICROMANIPULATOR

非接触和非破坏性的操纵技术，如液体中微米级颗粒的输送、捕获和分离，目前在生物技术、微机械加工和微流体领域引起了极大的兴趣。以前我们已经开发了一种新型的激光超声微操作器(Lumm)，其中声辐射力和光辐射力都用作互补的非接触力。在这项研究中，我们研究了一种在Windows PC上使用图形用户界面(GUI)的高性能Lumm以及操纵液滴的可能性。本研究从2001年4月至2002年3月进行了为期两年的研究。1)为了提高实验流明的性能，在PC机上利用Windows 98下的图形用户界面实现了显微镜图像的采集和机械工作台的GPIB控制。然而，超声波功率和激光功率…的GPIB控制2)利用最近在电子领域引起关注的紫外光固化胶粘剂技术，实验制备了小尺寸楔形换能器作为超声波操纵的超声源。在5.48 MHz工作频率下，获得了最小插入损耗为25.3d B、3d B带宽为9%的良好特性。3)利用楔形换能器快速去除大量颗粒中不必要的颗粒，实现了只捕获特定微米级颗粒的实验。提高楔形换能器的工作频率以减少对换能器的电气输入功率是未来的问题。4)近年来，人们注意到在几厘米大小的玻璃和塑料基板上集成了各种过程，如传输和反应、分离、纯化、检测等过程，即所谓的片上实验室(Lab-on-a-Chip，LOC)。为了实现测井，有必要发展微流控技术，不需要复杂的泵和管，就可以操纵极少量的液滴(0.5μL-60μL)。然后，在与激光超声波微操作器(Lumm)集成的情况下，测试了利用6 MHz的板波(Lamb波)对液滴的操纵。结果表明，这种操作是可能的。这些结果的一部分已在INST中给出。日本电气工程师协会第32届EM研讨会。未发表的结果将在不久的将来公布。本研究将在本次研究成果的基础上继续进行。较少

Noncontact and nondestructive techniques for manipulation, such as transportation, trapping, and separation of micron-sized particles in liquid, are currently of considerable interest in areas of biotechnology, micromachining and microfuidics. Previously we have developed a new laser ultrasonic micromanipulator (LUMM) in which the acoustic radiation force and optical radiation force are both used as complementary noncontact forces. In this study, we have studied a high performance LUMM using a graphical user interface (GUI) on a Windows PC and the possibility of the liquid droplet to manipulation. This study was carried out for 2 years from April, 2001 by March, 2002. The following results and prospects were obtained.1) For the purpose of the performance enhancement of the experimental LUMM, capturing of the microscope image and GPIB control of the mechanical stage using GUI on Windows 98 in a PC were constructed successfully. However, the GPIB control of ultrasonic power and laser pow … More er became future problem.2) Using UV curing adhesive technology noticed in the electronic field recently, the wedge transducer of the small shape as ultrasonic source of the ultrasonic wave manipulation was produced experimentally. At the 5.48MHz operating frequency, the good characteristic of minimum insertion loss of 25.3dB and 3dB bandwidth of 9% was obtained.3) The experiment of trapping only the specific microns-sized particle by the removal of unnecessary particles using the wedge transducer from large number of particle quickly was carried out successfully. It is future problem to increase the operating frequency of the wedge transducer in order to reduce the electrical input power to the transducer.4) Recently, various processes such as transport and reaction, separation, purification, detection that are an element of the chemical process are integrated on glass and plastic substrate of several centimeter sizes are noticed, and so called Lab-on-a-Chip (LOC). It is indispensable to develop microfluidic technology which manipulates liquid droplet of a very small quantity (0.5μl-60μl) without requiring complicated pump and tube in order to realize LOG. Then, the manipulation of the droplet using plate wave (Lamb wave) of the 6MHz was tested assuming the integration to laser ultrasonic wave micromanipulator (LUMM). The result showed that the manipulation was possible.The part of these results was presented in the Inst. of Electrical Engineers of Japan 32nd EM symposium. The unpublished results will be presented in near future. The research will be continued on the basis of the result obtained through this study. Less